ArticleStructure (London, England : 1993)2016
Acoustic Injectors for Drop-On-Demand Serial Femtosecond Crystallography.
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Article in Structure (London, England : 1993), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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47 citing papers in PubMed.
- Compact tape-driven sample delivery system for serial femtosecond crystallography.Journal of applied crystallography · 2026Article
- Minimized sample consumption for time-resolved serial crystallography applied to the redox cycle of human NQO1.Communications chemistry · 2026Article
- Microfluidic Devices for High-Throughput In Situ Serial Crystallography.Advances in biochemical engineering/biotechnology · 2026Review
- Sample delivery methods for protein X-ray crystallography with a special focus on sample consumption.Nature communications · 2025Review
- Standard Sample Preparation for Serial Femtosecond Crystallography.Biomolecules · 2025Review
- Superstability of micrometre jets surrounded by a polymeric shell.Journal of applied crystallography · 2025Article
- Review
- Macromolecular crystallography and biology at the Linac Coherent Light Source.Journal of synchrotron radiation · 2025Article
- Kilohertz droplet-on-demand serial femtosecond crystallography at the European XFEL station FXE.Structural dynamics (Melville, N.Y.) · 2024Article
- Article
- Characterization of Biological Samples Using Ultra-Short and Ultra-Bright XFEL Pulses.Advances in experimental medicine and biology · 2024Article
- A multi-reservoir extruder for time-resolved serial protein crystallography and compound screening at X-ray free-electron lasers.Nature communications · 2023Article
- Heterogeneity in M. tuberculosis β-lactamase inhibition by Sulbactam.Nature communications · 2023Article
- Modular droplet injector for sample conservation providing new structural insight for the conformational heterogeneity in the disease-associated NQO1 enzyme.Lab on a chip · 2023Article
- Emerging Time-Resolved X-Ray Diffraction Approaches for Protein Dynamics.Annual review of biophysics · 2023Review
- Structural evidence for intermediates during ONature · 2023Article
- Growing and making nano- and microcrystals.Nature protocols · 2023Review
- Inspection on the Mechanism of SARS-CoV-2 Inhibition by Penciclovir: A Molecular Dynamic Study.Molecules (Basel, Switzerland) · 2022Article
- Electrically stimulated droplet injector for reduced sample consumption in serial crystallography.Biophysical reports · 2022Article
- Acoustic Focusing of Protein Crystals for In-Line Monitoring and Up-Concentration during Serial Crystallography.Analytical chemistry · 2022Article
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Authors and funding
50 authors.
Christian G RoesslerPhoton Sciences Division, Brookhaven National Laboratory, Upton, NY 11973-5000, USA.
Rakhi AgarwalBiosciences Department, Brookhaven National Laboratory, Upton, NY 11973-5000, USA.
Marc AllairePhoton Sciences Division, Brookhaven National Laboratory, Upton, NY 11973-5000, USA. Electronic address: mallaire@lbl.gov.
Roberto Alonso-MoriLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Babak AndiPhoton Sciences Division, Brookhaven National Laboratory, Upton, NY 11973-5000, USA.
José F R BachegaCentro de Biotecnologia Molecular Estrutural, Instituto de Física de São Carlos, Universidade de São Paulo, Caixa Postal 369, São Carlos, CEP: 13560-970, Brazil.
Martin BommerInstitut für Biologie, Humboldt-Universität zu Berlin, D-10099 Berlin, Germany.
Aaron S BrewsterPhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720-8177, USA.
Michael C BrowneLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Ruchira ChatterjeePhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720-8177, USA.
Eunsun ChoDepartment of Chemistry, Boston University, Boston, MA 02215-2521, USA.
Aina E CohenStanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Matthew CowanPhoton Sciences Division, Brookhaven National Laboratory, Upton, NY 11973-5000, USA.
Sammy DatwaniLabcyte Inc., Sunnyvale, CA 94089, USA.
Victor L DavidsonBurnett School of Biomedical Sciences, University of Central Florida, Orlando, FL 32816-2364, USA.
Jim DefeverLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Brent EatonLabcyte Inc., Sunnyvale, CA 94089, USA.
Richard EllsonLabcyte Inc., Sunnyvale, CA 94089, USA.
Yiping FengLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Lucien P GhislainLabcyte Inc., Sunnyvale, CA 94089, USA.
James M GlowniaLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Guangye HanPhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720-8177, USA.
Johan HattnePhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720-8177, USA.
Julia HellmichMax-Volmer-Laboratorium für Biophysikalische Chemie, Technische Universität, D-10623 Berlin, Germany; Institut für Biologie, Humboldt-Universität zu Berlin, D-10099 Berlin, Germany.
Annie HérouxPhoton Sciences Division, Brookhaven National Laboratory, Upton, NY 11973-5000, USA.
Mohamed IbrahimInstitut für Biologie, Humboldt-Universität zu Berlin, D-10099 Berlin, Germany.
Jan KernPhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720-8177, USA; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Anthony KuczewskiPhoton Sciences Division, Brookhaven National Laboratory, Upton, NY 11973-5000, USA.
Henrik T LemkeLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Pinghua LiuDepartment of Chemistry, Boston University, Boston, MA 02215-2521, USA.
Lars MajlofLabcyte Inc., Sunnyvale, CA 94089, USA.
William M McClintockLabcyte Inc., Sunnyvale, CA 94089, USA.
Stuart MyersPhoton Sciences Division, Brookhaven National Laboratory, Upton, NY 11973-5000, USA.
Silke NelsenLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Joe OlechnoLabcyte Inc., Sunnyvale, CA 94089, USA.
Allen M OrvillePhoton Sciences Division, Brookhaven National Laboratory, Upton, NY 11973-5000, USA; Biosciences Department, Brookhaven National Laboratory, Upton, NY 11973-5000, USA. Electronic address: allen.orville@diamond.ac.uk.
Nicholas K SauterPhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720-8177, USA.
Alexei S SoaresPhoton Sciences Division, Brookhaven National Laboratory, Upton, NY 11973-5000, USA. Electronic address: soares@bnl.gov.
S Michael SoltisStanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Heng SongDepartment of Chemistry, Boston University, Boston, MA 02215-2521, USA.
Richard G StearnsLabcyte Inc., Sunnyvale, CA 94089, USA.
Rosalie TranPhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720-8177, USA.
Yingssu TsaiLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA; Department of Chemistry, Stanford University, Stanford, CA 94305-4401, USA.
Monarin UervirojnangkoornPhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720-8177, USA.
Carrie M WilmotDepartment of Biochemistry, Molecular Biology & Biophysics, University of Minnesota, Minneapolis, MN 55455, USA.
Vittal YachandraPhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720-8177, USA.
Junko YanoPhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720-8177, USA.
Erik T YuklDepartment of Biochemistry, Molecular Biology & Biophysics, University of Minnesota, Minneapolis, MN 55455, USA.
Diling ZhuLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Athina ZouniInstitut für Biologie, Humboldt-Universität zu Berlin, D-10099 Berlin, Germany.
Funding
X-RAY STRUCTURE OF RIFM PROTEIN FROM THE BIOSYNTHESIS PATHWAY OF THE ANSAMYCIN AP41RR012408 · NCRR · BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB · PI MARIUZZA, ROY A · 1998 to 2011
$32.2MX-ray Absorption Spectroscopy (XAS) pp.711-759P41GM103393 · NIGMS · STANFORD UNIVERSITY · PI HODGSON, KEITH O · 2012 to 2019
$30.6MLife Science and Biomedical technology Research center at NSLS-II LSBRP41GM111244 · NIGMS · BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB · PI MCSWEENEY, SEAN · 2014 to 2018
$16.4MTaking Snapshots of the Water-Oxidation Reaction by the Mn4Ca Oxygen-Evolving Complex Using X-ray Crystallography and SpectroscopyR01GM055302 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI YACHANDRA, VITTAL · 1997 to 2022
$9.1MMacromolecular Crystallography at the National Synchrotron Light SourceP41GM103473 · NIGMS · BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB · PI SWEET, ROBERT M · 2012 to 2013
$4.9MBiological Spectroscopy and Crystallography Using an X-ray Free Electron Laser - Supplemental EquipmentR01GM110501 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI Junko Yano · 2014 to 2026
$4.8MCatalysis and biogenesis in methylamine dehydrogenaseR01GM066569 · NIGMS · UNIVERSITY OF MINNESOTA TWIN CITIES · PI WILMOT, CAROLINE MARY · 2002 to 2015
$4.0MStructure Function & Biosynthesis of Respiratory EnzymesR37GM041574 · NIGMS · UNIVERSITY OF MISSISSIPPI MED CTR · PI DAVIDSON, VICTOR L · 2009 to 2018
$3.4MSTRUCTURE/FUNCTION &BIOSYNTHESIS OF RESPIRATORY ENZYMESR01GM041574 · NIGMS · UNIVERSITY OF MISSISSIPPI MEDICAL CENTER · PI DAVIDSON, VICTOR L · 1988 to 2008
$2.7MRealizing New Horizons in X-ray Crystallography Data ProcessingR01GM095887 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI SAUTER, NICHOLAS K · 2011 to 2014
$1.5MTowards real-time XFEL data reduction with CCTBXR01GM102520 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI SAUTER, NICHOLAS K · 2012 to 2015
$1.4MSUPPORT FOR CONTINUOUS RESEARCH EXCELLENCES06GM008003 · NIGMS · SOUTHEASTERN OKLAHOMA STATE UNIVERSITY · PI PAIVA, NANCY · 1989 to 2007
$1.3MHoward Hughes Medical InstituteNCRR NIH HHS 2-P41-RR012408NCRR NIH HHS P41 RR012408NIGMS NIH HHS 8P41GM103473-16NIGMS NIH HHS F32 GM097779NIGMS NIH HHS GM055302NIGMS NIH HHS GM095887NIGMS NIH HHS GM102520NIGMS NIH HHS GM110501NIGMS NIH HHS NIH 5R01 GM066569-11NIGMS NIH HHS NIH 5R37 GM041574-26NIGMS NIH HHS NIH F32 GM097779-03NIGMS NIH HHS P41 GM103393NIGMS NIH HHS P41GM103393NIGMS NIH HHS P41 GM103473NIGMS NIH HHS P41 GM111244NIGMS NIH HHS R01 GM041574NIGMS NIH HHS R01 GM055302NIGMS NIH HHS R01 GM066569NIGMS NIH HHS R01 GM095887NIGMS NIH HHS R01 GM102520NIGMS NIH HHS R01 GM110501NIGMS NIH HHS R37 GM041574NIGMS NIH HHS R56 GM055302NIGMS NIH HHS S06 GM008003NIGMS NIH HHS Y1GM008003
Abstract
X-ray free-electron lasers (XFELs) provide very intense X-ray pulses suitable for macromolecular crystallography. Each X-ray pulse typically lasts for tens of femtoseconds and the interval between pulses is many orders of magnitude longer. Here we describe two novel acoustic injection systems that use focused sound waves to eject picoliter to nanoliter crystal-containing droplets out of microplates and into the X-ray pulse from which diffraction data are collected. The on-demand droplet delivery is synchronized to the XFEL pulse scheme, resulting in X-ray pulses intersecting up to 88% of the droplets. We tested several types of samples in a range of crystallization conditions, wherein the overall crystal hit ratio (e.g., fraction of images with observable diffraction patterns) is a function of the microcrystal slurry concentration. We report crystal structures from lysozyme, thermolysin, and stachydrine demethylase (Stc2). Additional samples were screened to demonstrate that these methods can be applied to rare samples.
Indexed as
AcousticsCrystallography, X-RayEnzymesModels, MolecularMuramidaseProtein ConformationThermolysinEnzymesMuramidaseThermolysin
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