ArticleScientific reports2016
Lighting Up Clostridium Difficile: Reporting Gene Expression Using Fluorescent Lov Domains.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 68 citations in OpenAlex.
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- Analysis of Essential Genes inbioRxiv : the preprint server for biology · 2025Article
- Molecular dissection of Class A PBP function uncovers novel features of the non-canonicalbioRxiv : the preprint server for biology · 2025Article
- Microbial single-cell applications under anoxic conditions.Applied and environmental microbiology · 2024Review
- Rational Design of a Circularly Permuted Flavin-Based Fluorescent Protein.Chembiochem : a European journal of chemical biology · 2024Article
- Fluorescent Protein-Based Sensors for Detecting Essential Metal Ions across the Tree of Life.ACS sensors · 2024Review
- Development and utilization of new OMicrobiology spectrum · 2024Article
- A monoclonal antibody collection for C. difficile typing ?Gut pathogens · 2024Article
- Bacterial metabolites influence the autofluorescence ofFrontiers in microbiology · 2024Article
- Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Anaerobic fluorescent reporters for live imaging ofFrontiers in microbiology · 2023Article
- A fluorescent reporter system for anaerobic thermophiles.Frontiers in bioengineering and biotechnology · 2023Article
- Mutant Flavin-Based Fluorescent Protein Sensors for Detecting Intracellular Zinc and Copper inACS sensors · 2022Article
- Article
- Practical observations on the use of fluorescent reporter systems in Clostridioides difficile.Antonie van Leeuwenhoek · 2022Article
- Enhanced small green fluorescent proteins as a multisensing platform for biosensor development.Frontiers in bioengineering and biotechnology · 2022Article
- New Orange Ligand-Dependent Fluorescent Reporter for Anaerobic Imaging.ACS chemical biology · 2021Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
The uses of fluorescent reporters derived from green fluorescent protein have proved invaluable for the visualisation of biological processes in bacteria grown under aerobic conditions. However, their requirement for oxygen has limited their application in obligate anaerobes such as Clostridium difficile. Fluorescent proteins derived from Light, Oxygen or Voltage sensing (LOV) domains have been shown to bridge this limitation, but their utility as translational fusions to monitor protein expression and localisation in a strict anaerobic bacterium has not been reported. Here we demonstrate the utility of phiLOV in three species of Clostridium and its application as a marker of real-time protein translation and dynamics through genetic fusion with the cell division protein, FtsZ. Time lapse microscopy of dividing cells suggests that Z ring assembly arises through the extension of the FtsZ arc starting from one point on the circumference. Furthermore, through incorporation of phiLOV into the flagella subunit, FliC, we show the potential of bacterial LOV-based fusion proteins to be successfully exported to the extracellular environment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.