Evidence map›Paper›PMID 26996201›Full record

ArticleNature communications2016

Open-source image reconstruction of super-resolution structured illumination microscopy data in ImageJ.

Marcel Müller, Viola Mönkemöller, Simon Hennig, Wolfgang Hübner, Thomas Huser

Abstract read
In one paragraph

Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 106 papers.

0numbers the graph read from it
0cells of the map it votes in
106citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

106 citing papers in PubMed.

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  12. Biomedical optics express · 2025
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46 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Marcel MüllerBiomolecular Photonics, Department of Physics, University of Bielefeld, 33615 Bielefeld, Germany.
Viola MönkemöllerBiomolecular Photonics, Department of Physics, University of Bielefeld, 33615 Bielefeld, Germany.
Simon HennigBiomolecular Photonics, Department of Physics, University of Bielefeld, 33615 Bielefeld, Germany.
Wolfgang HübnerBiomolecular Photonics, Department of Physics, University of Bielefeld, 33615 Bielefeld, Germany.
Thomas HuserBiomolecular Photonics, Department of Physics, University of Bielefeld, 33615 Bielefeld, Germany.ORCID http://orcid.org/0000-0003-2348-7416

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Super-resolved structured illumination microscopy (SR-SIM) is an important tool for fluorescence microscopy. SR-SIM microscopes perform multiple image acquisitions with varying illumination patterns, and reconstruct them to a super-resolved image. In its most frequent, linear implementation, SR-SIM doubles the spatial resolution. The reconstruction is performed numerically on the acquired wide-field image data, and thus relies on a software implementation of specific SR-SIM image reconstruction algorithms. We present fairSIM, an easy-to-use plugin that provides SR-SIM reconstructions for a wide range of SR-SIM platforms directly within ImageJ. For research groups developing their own implementations of super-resolution structured illumination microscopy, fairSIM takes away the hurdle of generating yet another implementation of the reconstruction algorithm. For users of commercial microscopes, it offers an additional, in-depth analysis option for their data independent of specific operating systems. As a modular, open-source solution, fairSIM can easily be adapted, automated and extended as the field of SR-SIM progresses.

Identifiers

PMID26996201
PMCPMC4802170

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.