ArticleJournal of pediatric surgery2016
Long-term renewable human intestinal epithelial stem cells as monolayers: A potential for clinical use.
Article in Journal of pediatric surgery, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed.
- Human mesofluidic intestinal model for studying transport of drug carriers and bacteria through a live mucosal barrier.Lab on a chip · 2025Article
- The Use of Gut Organoids: To Study the Physiology and Disease of the Gut Microbiota.Journal of cellular and molecular medicine · 2025Review
- Matrix-free human 2D organoids recapitulate duodenal barrier and transport properties.BMC biology · 2025Article
- Listen to Your Gut: Key Concepts for Bioengineering Advanced Models of the Intestine.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Establishment and characterization of adult human gastric epithelial progenitor-like cell lines.Cell proliferation · 2023Article
- From Patient Material to New Discoveries: a Methodological Review and Guide for Intestinal Stem Cell Researchers.Stem cell reviews and reports · 2022Review
- Metabolic model of necrotizing enterocolitis in the premature newborn gut resulting from enteric dysbiosis.Frontiers in pediatrics · 2022Article
- Navigating Through Cell-Based In vitro Models Available for Prediction of Intestinal Permeability and Metabolism: Are We Ready for 3D?The AAPS journal · 2021Article
- Drivers of transcriptional variance in human intestinal epithelial organoids.Physiological genomics · 2021Article
- Current knowledge on the multiform reconstitution of intestinal stem cell niche.World journal of stem cells · 2021Review
- A bioengineering perspective on modelling the intestinal epithelial physiology in vitro.Nature communications · 2020Review
- Histo-blood group antigens of glycosphingolipids predict susceptibility of human intestinal enteroids to norovirus infection.The Journal of biological chemistry · 2020Article
- Mechanical Stimuli Affect Escherichia coli Heat-Stable Enterotoxin-Cyclic GMP Signaling in a Human Enteroid Intestine-Chip Model.Infection and immunity · 2020Article
- Self-organized intestinal epithelial monolayers in crypt and villus-like domains show effective barrier function.Scientific reports · 2019Article
- Primary Cell-Derived Intestinal Models: Recapitulating Physiology.Trends in biotechnology · 2019Review
- Intestinal epithelial replacement by transplantation of cultured murine and human cells into the small intestine.PloS one · 2019Article
- Review
- Mechanisms for intestinal regeneration.Current opinion in pediatrics · 2018Review
- Towards a defined ECM and small molecule based monolayer culture system for the expansion of mouse and human intestinal stem cells.Biomaterials · 2018Article
- Monolayer culture of intestinal epithelium sustains Lgr5Cell discovery · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
purposeCurrent culture schema for human intestinal stem cells (hISCs) frequently rely on a 3D culture system using Matrigel™, a laminin-rich matrix derived from murine sarcoma that is not suitable for clinical use. We have developed a novel 2D culture system for the in vitro expansion of hISCs as an intestinal epithelial monolayer without the use of Matrigel.
methodsCadaveric duodenal samples were processed to isolate intestinal crypts from the mucosa. Crypts were cultured on a thin coat of type I collagen or laminin. Intestinal epithelial monolayers were supported with growth factors to promote self-renewal or differentiation of the hISCs. Proliferating monolayers were sub-cultured every 4-5days.
resultsIntestinal epithelial monolayers were capable of long-term cell renewal. Less differentiated monolayers expressed high levels of gene marker LGR5, while more differentiated monolayers had higher expressions of CDX2, MUC2, LYZ, DEF5, and CHGA. Furthermore, monolayers were capable of passaging into a 3D culture system to generate spheroids and enteroids.
conclusionThis 2D system is an important step to expand hISCs for further experimental studies and for clinical cell transplantation. LEVEL OF EVIDENCE: 1 Experimental.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.