Evidence map›Paper›PMID 26995495›Full record

ArticleMolecular genetics and genomics : MGG2016

Molecular characterization of Sp110 gene in pigs.

Li-Zu Li, Qiu-Shi Wang, Li-Xin Han, Jin-Kui Wang, Si-Yu Shao, Liang Wang, Di Liu, Xiu-Qin Yang

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular genetics and genomics : MGG, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Li-Zu LiCollege of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
Qiu-Shi WangCollege of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
Li-Xin HanCollege of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
Jin-Kui WangCollege of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
Si-Yu ShaoCollege of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
Liang WangInstitute of Animal Husbandry, Heilongjiang Academy of Agricultural Sciences, Harbin, China.
Di LiuInstitute of Animal Husbandry, Heilongjiang Academy of Agricultural Sciences, Harbin, China. liudi1963@163.com.ORCID http://orcid.org/0000-0002-6618-6675
Xiu-Qin YangCollege of Animal Science and Technology, Northeast Agricultural University, Harbin, China. xiuqin163@163.com.
Northeast Agricultural University · CNAnimal Science Research Institute · CNHeilongjiang Academy of Sciences · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Speckled 110 kDa (Sp110) plays an important role in infectious diseases, as revealed by studies in humans. However, little is known regarding porcine Sp110. To elucidate its potential role in porcine resistance to viral diseases, here, the complete coding sequence of porcine Sp110 gene and its 26 alternatively spliced isoforms were isolated using reverse transcription (RT)-polymerase chain reaction (PCR), and another seven splicing patterns were obtained using a minigene construct. Subcellular distribution of 11 representative isoforms was characterized in PK-15 cells transiently transfected with their respective GFP fusion constructs, and only isoforms (R and V) bearing all functional domains were localized in nucleus, indicating all the other isoforms lose normal functions of Sp110 owing to alternative splicing. Real-time quantitative PCR and competitive RT-PCR showed that both isoforms R and V had similar tissue expression profile, half-life and response to poly(I:C), a synthetic analog of viral double-stranded RNA, while the longer one (isoform R) was transcribed at a higher level. The results indicated that porcine Sp110 has a role in viral infection and that isoform R is the dominant active form. Overall the data provide potential resource for molecular breeding of pig resistant to diseases and contributes to breeding pigs resistant to viral infection.

Indexed as

Alternative SplicingAnimalsCell LineCloning, MolecularGene Expression RegulationNuclear ProteinsPoly I-CPolynucleotidesSwineSwine, MiniatureTissue DistributionNuclear ProteinsPoly I-CPolynucleotidesAlternative splicingExpressionPigSp110Subcellular localization

Identifiers

PMID26995495
OpenAlexW2310173839

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.