ArticleCurrent protocols in chemical biology2016
RNA Imaging with Dimeric Broccoli in Live Bacterial and Mammalian Cells.
Article in Current protocols in chemical biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 49 citations in OpenAlex.
- Engineering a Synthetic Fluorogenic RNA Switch for Oxidized Guanine.Journal of fluorescence · 2026Article
- Fluorescence-Activated Cell Sorting Enables Rapid Screening of RNA Polymerase-Promoter Interaction in E. coli.Biochemistry · 2026Article
- Burden-aware feedback control of microbial consortia.Nature communications · 2026Article
- Identification of Phage RNA Polymerases That Minimize Double-Stranded RNA By-Product Formation and Their Characterization via In Vitro Transcription.Microorganisms · 2026Article
- Programmable and Switchable RNA Scaffolds for Synthetic Condensate Engineering in Mammalian Cells.bioRxiv : the preprint server for biology · 2026Article
- Evaluating DFHBI-Responsive RNA Light-Up Aptamers as Fluorescent Reporters for Gene Expression.ACS synthetic biology · 2023Article
- Targeted RNA condensation in living cells via genetically encodable triplet repeat tags.Nucleic acids research · 2023Article
- Genetically encoded RNA-based sensors with Pepper fluorogenic aptamer.Nucleic acids research · 2023Article
- Targeted RNA Condensation in Living Cells via Genetically Encodable Triplet Repeat Tags.bioRxiv : the preprint server for biology · 2023Article
- Genetically Encoded RNA-Based Bioluminescence Resonance Energy Transfer (BRET) Sensors.ACS sensors · 2023Article
- Cell-Free Gene Expression Dynamics in Synthetic Cell Populations.ACS synthetic biology · 2022Article
- Traditional protocols and optimization methods lead to absent expression in a mycoplasma cell-free gene expression platform.Synthetic biology (Oxford, England) · 2022Article
- Sensitive fluorescence detection of SARS-CoV-2 RNA in clinical samples via one-pot isothermal ligation and transcription.Nature biomedical engineering · 2020Article
- A protein-independent fluorescent RNA aptamer reporter system for plant genetic engineering.Nature communications · 2020Article
- Use of Genetically Encoded Fluorescent Aptamers for Visualization of Mycobacterium tuberculosis Small RNA MTS1338 in Infected Macrophages.Doklady. Biochemistry and biophysics · 2020Article
- Fluorophore-Promoted RNA Folding and Photostability Enables Imaging of Single Broccoli-Tagged mRNAs in Live Mammalian Cells.Angewandte Chemie (International ed. in English) · 2020Article
- The chemistry and applications of RNA 2'-OH acylation.Nature reviews. Chemistry · 2020Article
- A multicolor riboswitch-based platform for imaging of RNA in live mammalian cells.Methods in enzymology · 2020Article
- Genetically Encoded Ratiometric RNA-Based Sensors for Quantitative Imaging of Small Molecules in Living Cells.Angewandte Chemie (International ed. in English) · 2019Article
- Highly efficient expression of circular RNA aptamers in cells using autocatalytic transcripts.Nature biotechnology · 2019Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
RNA spatial dynamics play a crucial role in cell physiology, and thus the ability to monitor RNA localization in live cells can provide insight into important biological problems. This unit focuses on imaging RNAs using an RNA mimic of GFP. This approach relies on an RNA aptamer called dimeric Broccoli, which binds to and switches on the fluorescence of DFHBI, a small molecule mimicking the fluorophore in GFP. Dimeric Broccoli is tagged to heterologously expressed RNAs and, upon DFHBI binding, the fluorescent signal of dimeric Broccoli reports the transcript's localization in cells. This protocol describes the process of validating the fluorescence of dimeric Broccoli--labeled transcripts in vitro and in cells, flow cytometry analysis to determine overall fluorescence levels in cells, and fluorescence imaging in bacterial and mammalian cells. Overall, the protocol should be useful for researchers seeking to image high-abundance RNAs, such as those transcribed off the T7 promoter in bacteria or off Pol III--dependent promoters in mammalian cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.