ArticleJournal of experimental botany2016
Photorespiratory glycolate oxidase is essential for the survival of the red alga Cyanidioschyzon merolae under ambient CO2 conditions.
Article in Journal of experimental botany, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- The mosaic of aquatic photorespiration: evolution, diversity, and global biogeochemical impacts.The New phytologist · 2026Review
- Optimization of Ultrasound-Assisted Extraction Process for Silkworm (Molecules (Basel, Switzerland) · 2025Article
- Modeling with uncertainty quantification reveals the essentials of a non-canonical algal carbon-concentrating mechanism.Plant physiology · 2025Article
- Removal of phosphoglycolate in hyperthermophilic archaea.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- The carbon-concentrating mechanism of the extremophilic red microalga Cyanidioschyzon merolae.Photosynthesis research · 2023Article
- Mixotrophic growth of the extremophile Galdieria sulphuraria reveals the flexibility of its carbon assimilation metabolism.The New phytologist · 2021Article
- Photosynthesis of the Cyanidioschyzon merolae cells in blue, red, and white light.Photosynthesis research · 2021Article
- Evolution of Photorespiratory Glycolate Oxidase among Archaeplastida.Plants (Basel, Switzerland) · 2020Article
- Bioengineering of Microalgae: Recent Advances, Perspectives, and Regulatory Challenges for Industrial Application.Frontiers in bioengineering and biotechnology · 2020Review
- Faster Removal of 2-Phosphoglycolate through Photorespiration Improves Abiotic Stress Tolerance of Arabidopsis.Plants (Basel, Switzerland) · 2019Article
- Redox-Regulation of Photorespiration through Mitochondrial Thioredoxin o1.Plant physiology · 2019Article
- Glycolate from microalgae: an efficient carbon source for biotechnological applications.Plant biotechnology journal · 2019Article
- Article
- Comparative analysis of strategies to prepare electron sinks in aquatic photoautotrophs.Photosynthesis research · 2019Article
- Nitrate or ammonium: Influences of nitrogen source on the physiology of a green alga.Ecology and evolution · 2019Article
- Efficient 2-phosphoglycolate degradation is required to maintain carbon assimilation and allocation in the C4 plant Flaveria bidentis.Journal of experimental botany · 2019Article
- The PhotorespiratoryFrontiers in plant science · 2018Article
- Oxidation of P700 Ensures Robust Photosynthesis.Frontiers in plant science · 2018Review
- The ancestors of diatoms evolved a unique mitochondrial dehydrogenase to oxidize photorespiratory glycolate.Photosynthesis research · 2017Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Photorespiration is essential for all organisms performing oxygenic photosynthesis. The evolution of photorespiratory metabolism began among cyanobacteria and led to a highly compartmented pathway in plants. A molecular understanding of photorespiration in eukaryotic algae, such as glaucophytes, rhodophytes, and chlorophytes, is essential to unravel the evolution of this pathway. However, mechanistic detail of the photorespiratory pathway in red algae is scarce. The unicellular red alga Cyanidioschyzon merolae represents a model for the red lineage. Its genome is fully sequenced, and tools for targeted gene engineering are available. To study the function and importance of photorespiration in red algae, we chose glycolate oxidase (GOX) as the target. GOX catalyses the conversion of glycolate into glyoxylate, while hydrogen peroxide is generated as a side-product. The function of the candidate GOX from C. merolae was verified by the fact that recombinant GOX preferred glycolate over L-lactate as a substrate. Yellow fluorescent protein-GOX fusion proteins showed that GOX is targeted to peroxisomes in C. merolae The GOX knockout mutant lines showed a high-carbon-requiring phenotype with decreased growth and reduced photosynthetic activity compared to the wild type under ambient air conditions. Metabolite analyses revealed glycolate and glycine accumulation in the mutant cells after a shift from high CO2 conditions to ambient air. In summary, or results demonstrate that photorespiratory metabolism is essential for red algae. The use of a peroxisomal GOX points to a high photorespiratory flux as an ancestral feature of all photosynthetic eukaryotes.
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