ArticlePlant physiology2016
The Cytoplasmic Carbonic Anhydrases βCA2 and βCA4 Are Required for Optimal Plant Growth at Low CO2.
Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 106 citations in OpenAlex.
- Crosstalk between β-carbonic anhydrases and PsbS in the regulation of photosynthesis and stress tolerance in Arabidopsis.Journal of experimental botany · 2026Article
- Parsing the stepwise molecular evolution of C4-associated β-carbonic anhydrase in the genus Flaveria.Plant physiology · 2026Article
- Carbonic anhydrases contribute to mitochondrial function, conidial development, and pathogenicity ofApplied and environmental microbiology · 2026Article
- A guard cell carbonic anhydrase binds and regulates SLAC1 separate from its catalytic activity.Nature communications · 2026Article
- Transcriptomic insights into gibberellin-3-mediated regulation of bud dormancy and metabolic transition in pitaya (Hylocereus polyrhizus).Plant cell reports · 2025Article
- Effect of the Absence of α Carbonic Anhydrase 2 on the PSII Light-Harvesting Complex Size inPlants (Basel, Switzerland) · 2025Article
- Effects of DCA gene overexpression in transgenic Dunaliella parva.Antonie van Leeuwenhoek · 2024Article
- Expression and localization of two β-carbonic anhydrases inFrontiers in plant science · 2024Article
- Root-based inorganic carbon uptake increases the growth ofFrontiers in plant science · 2024Article
- Rewiring of primary metabolism for ammonium recycling under short-term low COFrontiers in plant science · 2024Article
- Carbonic anhydrases in the cell wall and plasma membrane ofFrontiers in molecular biosciences · 2024Article
- The Carbonic Anhydrase βCA1 Functions in PopW-Mediated Plant Defense Responses in Tomato.International journal of molecular sciences · 2023Article
- Exploring the potential of Δ17O in CO2 for determining mesophyll conductance.Plant physiology · 2023Article
- The Gynandropsis gynandra genome provides insights into whole-genome duplications and the evolution of C4 photosynthesis in Cleomaceae.The Plant cell · 2023Article
- Genome-wide identification, evolution, and expression analysis of carbonic anhydrases genes in soybean (Glycine max).Functional & integrative genomics · 2023Review
- Response of the organellar and nuclear (post)transcriptomes of Arabidopsis to drought.Frontiers in plant science · 2023Article
- Encoded CPlant signaling & behavior · 2022Review
- The Role of Carbonic Anhydrase αCA4 in Photosynthetic Reactions inPlants (Basel, Switzerland) · 2022Article
- Identification of growth regulators using cross-species network analysis in plants.Plant physiology · 2022Article
- Arabidopsis plastid carbonic anhydrase βCA5 is important for normal plant growth.Plant physiology · 2022Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Carbonic anhydrases (CAs) are zinc metalloenzymes that interconvert CO2 and HCO3 (-) In plants, both α- and β-type CAs are present. We hypothesize that cytoplasmic βCAs are required to modulate inorganic carbon forms needed in leaf cells for carbon-requiring reactions such as photosynthesis and amino acid biosynthesis. In this report, we present evidence that βCA2 and βCA4 are the two most abundant cytoplasmic CAs in Arabidopsis (Arabidopsis thaliana) leaves. Previously, βCA4 was reported to be localized to the plasma membrane, but here, we show that two forms of βCA4 are expressed in a tissue-specific manner and that the two proteins encoded by βCA4 localize to two different regions of the cell. Comparing transfer DNA knockout lines with wild-type plants, there was no reduction in the growth rates of the single mutants, βca2 and βca4 However, the growth rate of the double mutant, βca2βca4, was reduced significantly when grown at 200 μL L(-1) CO2 The reduction in growth of the double mutant was not linked to a reduction in photosynthetic rate. The amino acid content of leaves from the double mutant showed marked reduction in aspartate when compared with the wild type and the single mutants. This suggests the cytoplasmic CAs play an important but not previously appreciated role in amino acid biosynthesis.
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