ArticleDevelopmental biology2017
Generation of a Xenopus laevis F1 albino J strain by genome editing and oocyte host-transfer.
Article in Developmental biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed, 20 citations in OpenAlex.
- Developing immortal cell lines fromOpen biology · 2022Article
- Review
- Oocyte Host-Transfer and Maternal mRNA Depletion Experiments inCold Spring Harbor protocols · 2018Article
- Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus.Journal of visualized experiments : JoVE · 2018Article
- High-efficiency non-mosaic CRISPR-mediated knock-in and indel mutation in F0Development (Cambridge, England) · 2017Article
- Expanding the genetic toolkit in Xenopus: Approaches and opportunities for human disease modeling.Developmental biology · 2017Review
- An optimized method for cryogenic storage of Xenopus sperm to maximise the effectiveness of research using genetically altered frogs.Theriogenology · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Completion of the Xenopus laevis genome sequence from inbred J strain animals has facilitated the generation of germline mutant X. laevis using targeted genome editing. In the last few years, numerous reports have demonstrated that TALENs are able to induce mutations in F0 Xenopus embryos, but none has demonstrated germline transmission of such mutations in X. laevis. In this report we used the oocyte host-transfer method to generate mutations in both tyrosinase homeologs and found highly-penetrant germline mutations; in contrast, embryonic injections yielded few germline mutations. We also compared the distribution of mutations in several F0 somatic tissues and germ cells and found that the majority of mutations in each tissue were different. These results establish that X. laevis J strain animals are very useful for generating germline mutations and that the oocyte host-transfer method is an efficient technique for generating mutations in both homeologs.
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Registered trials
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