ArticleGenome research2016
Sequence-targeted nucleosome sliding in vivo by a hybrid Chd1 chromatin remodeler.
Article in Genome research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
- ISW1a modulates cohesin distribution in centromeric and pericentromeric regions.Nucleic acids research · 2023Article
- Rapid and inexpensive preparation of genome-wide nucleosome footprints from model and non-model organisms.STAR protocols · 2021Article
- Ruler elements in chromatin remodelers set nucleosome array spacing and phasing.Nature communications · 2021Article
- Article
- Autoinhibitory elements of the Chd1 remodeler block initiation of twist defects by destabilizing the ATPase motor on the nucleosome.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Opposing chromatin remodelers control transcription initiation frequency and start site selection.Nature structural & molecular biology · 2019Article
- Repression of Divergent Noncoding Transcription by a Sequence-Specific Transcription Factor.Molecular cell · 2018Article
- A unified computational framework for modeling genome-wide nucleosome landscape.Physical biology · 2018Article
- The Latest Twists in Chromatin Remodeling.Biophysical journal · 2018Review
- Nanoscale dynamics of centromere nucleosomes and the critical roles of CENP-A.Nucleic acids research · 2018Article
- Understanding nucleosome dynamics and their links to gene expression and DNA replication.Nature reviews. Molecular cell biology · 2017Review
- The Sequence of Nucleosomal DNA Modulates Sliding by the Chd1 Chromatin Remodeler.Journal of molecular biology · 2017Article
- Sequence-specific targeting of chromatin remodelers organizes precisely positioned nucleosomes throughout the genome.BioEssays : news and reviews in molecular, cellular and developmental biology · 2017Review
- From profiles to function in epigenomics.Nature reviews. Genetics · 2017Review
- Article
- Article
- The Chd1 chromatin remodeler can sense both entry and exit sides of the nucleosome.Nucleic acids research · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
ATP-dependent chromatin remodelers regulate chromatin dynamics by modifying nucleosome positions and occupancy. DNA-dependent processes such as replication and transcription rely on chromatin to faithfully regulate DNA accessibility, yet how chromatin remodelers achieve well-defined nucleosome positioning in vivo is poorly understood. Here, we report a simple method for site-specifically altering nucleosome positions in live cells. By fusing the Chd1 remodeler to the DNA binding domain of the Saccharomyces cerevisiae Ume6 repressor, we have engineered a fusion remodeler that selectively positions nucleosomes on top of adjacent Ume6 binding motifs in a highly predictable and reproducible manner. Positioning of nucleosomes by the fusion remodeler recapitulates closed chromatin structure at Ume6-sensitive genes analogous to the endogenous Isw2 remodeler. Strikingly, highly precise positioning of single founder nucleosomes by either chimeric Chd1-Ume6 or endogenous Isw2 shifts phased chromatin arrays in cooperation with endogenous chromatin remodelers. Our results demonstrate feasibility of engineering precise nucleosome rearrangements through sequence-targeted chromatin remodeling and provide insight into targeted action and cooperation of endogenous chromatin remodelers in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.