ArticlePloS one2016
Helicobacter pylori Eradication Causes Perturbation of the Human Gut Microbiome in Young Adults.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT05449028 (Helicobacter Pylori Eradication Therapy in Portugal), which is not on this map. Cited by 68 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Helicobacter Pylori Eradication Therapy in Portugal: Prospective, Randomized, Blind and Multicentre Trial on the Efficacy of Quadruple Therapies and Their Clinical Impact, and Immunological and Gut Microbiota Changes
Who cites it
68 citing papers in PubMed, 2 syntheses or guidelines pooled it, 140 citations in OpenAlex.
- Worldwide research trends on theFrontiers in immunology · 2026Pooled it
- Association Between Infections and Risk of Ankylosing Spondylitis: A Systematic Review and Meta-Analysis.Frontiers in immunology · 2021Pooled it
- Evaluation of efficacy and safety ofFrontiers in immunology · 2024Trial
- Study of the effect of LactobacillusFrontiers in immunology · 2023Trial
- H. pylori eradication with antibiotic treatment causes changes in glucose homeostasis related to modifications in the gut microbiota.PloS one · 2019Trial
- Efficacy of compoundBMJ open · 2018Trial
- Trial
- Exploration of the Interaction Between Bismuth-Containing Quadruple Anti-Microorganisms · 2026Article
- Therapeutic Potential of Probiotics in the Modulation of Antibiotic Resistance inBiomedicines · 2026Review
- Effects of Helicobacter pylori infection on T cell activation markers and regulatory T cells in people with and without HIV infection in Central Ethiopia.European journal of medical research · 2026Article
- Concomitant versus reverse hybrid therapy for eradication of Helicobacter pylori infection-An open-label randomized controlled trial.Indian journal of gastroenterology : official journal of the Indian Society of Gastroenterology · 2025Article
- Caenorhabditis elegans microbiome members have combinatorial effects on host survival and fitness.Scientific reports · 2025Article
- Protocol for a randomised 'screen-and-treat'BMJ open · 2025Article
- Review
- Long-term changes in the gut microbiota before and after bismuth quadruple therapy or concomitant therapy in children with peptic ulcers receivingTranslational pediatrics · 2024Article
- Environmental and Genetic Determinants of Ankylosing Spondylitis.International journal of molecular sciences · 2024Review
- A nanoparticle-based sonodynamic therapy reduces Helicobacter pylori infection in mouse without disrupting gut microbiota.Nature communications · 2024Article
- Effect ofFrontiers in oncology · 2024Review
- Effect of probiotic supplementation combined with bismuth-containing quadruple therapy on gut microbiota duringFrontiers in nutrition · 2024Article
- Influence of Helicobacter pylori infection on risk of rheumatoid arthritis: a nationwide population-based study.Scientific reports · 2023Article
8 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAccumulating evidence shows that Helicobacter pylori protects against some metabolic and immunological diseases in which the development of these diseases coincide with temporal or permanent dysbiosis. The aim of this study was to assess the effect of H. pylori eradication on the human gut microbiome.
methodsAs part of the currently on-going ESSAY (Eradication Study in Stable Adults/Youths) study, we collected stool samples from 17 H. pylori-positive young adult (18-30 years-old) volunteers. The same cohort was followed up 6, 12 and 18 months-post H. pylori eradication. The impact of H. pylori on the human gut microbiome pre- and post-eradication was investigated using high throughput 16S rRNA gene (V3-V4 region) sequencing using the Illumina Miseq followed by data analysis using Qiime pipeline.
resultsWe compared the composition and diversity of bacterial communities in the fecal microbiome of the H. pylori-positive volunteers, before and after H. pylori eradication therapy. The 16S rRNA gene was sequenced at an average of 150,000-170,000 reads/sample. The microbial diversity were similar pre- and post-H. pylori eradication with no significant differences in richness and evenness of bacterial species. Despite that the general profile of the gut microbiome was similar pre- and post-eradication, some changes in the bacterial communities at the phylum and genus levels were notable, particularly the decrease in relative abundance of Bacterioidetes and corresponding increase in Firmicutes after H. pylori eradication. The significant increase of short-chain fatty acids (SCFA)-producing bacteria genera could also be associated with increased risk of metabolic disorders.
conclusionsOur preliminary stool metagenomics study shows that eradication of H. pylori caused perturbation of the gut microbiome and may indirectly affect the health of human. Clinicians should be aware of the effect of broad spectrum antibiotics used in H. pylori eradication regimen and be cautious in the clinical management of H. pylori infection, particularly in immunocompromised patients.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.