ArticleThe New phytologist2016
The endophytic symbiont Epichloë festucae establishes an epiphyllous net on the surface of Lolium perenne leaves by development of an expressorium, an appressorium-like leaf exit structure.
Article in The New phytologist, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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27 citing papers in PubMed, 78 citations in OpenAlex.
- Cdc25-Mediated Activation of the Small GTPase RasB Is Essential for Hyphal Fusion and Symbiotic Infection of Epichloë festucae.Molecular plant pathology · 2026Article
- Unveiling an asymmetric plant-fungal symbiosis: morphological, cytogenetic, and molecular characterization of a haploidIMA fungus · 2025Article
- Evaluating the Effects ofInsects · 2024Article
- The Impact of AbovegroundMicroorganisms · 2024Article
- Development of a molecular genetics and cell biology toolbox for the filamentous fungus Diplodia sapinea.PloS one · 2024Article
- Herbicide 2,4-dichlorophenoxyacetic acid interferes with MAP kinase signaling in Fusarium graminearum and is inhibitory to fungal growth and pathogenesis.Stress biology · 2023Article
- A broadly conserved fungal alcohol oxidase (AOX) facilitates fungal invasion of plants.Molecular plant pathology · 2023Article
- Article
- Cross-species transcriptomics identifies core regulatory changes differentiating the asymptomatic asexual and virulent sexual life cycles of grass-symbiotic Epichloë fungi.G3 (Bethesda, Md.) · 2022Article
- Epichloë scottii sp. nov., a new endophyte isolated from Melica uniflora is the missing ancestor of Epichloë disjuncta.IMA fungus · 2022Article
- Secreted Glycoside Hydrolase Proteins as Effectors and Invasion Patterns of Plant-Associated Fungi and Oomycetes.Frontiers in plant science · 2022Review
- Tracking Fungal Growth: Establishment of Arp1 as a Marker for Polarity Establishment and Active Hyphal Growth in Filamentous Ascomycetes.Journal of fungi (Basel, Switzerland) · 2021Article
- Global Changes in AsexualMicroorganisms · 2021Article
- Article
- Unraveling the sugar code: the role of microbial extracellular glycans in plant-microbe interactions.Journal of experimental botany · 2021Review
- Lolium perenne apoplast metabolomics for identification of novel metabolites produced by the symbiotic fungus Epichloë festucae.The New phytologist · 2020Article
- Molecular mechanisms in grass-Epichloë interactions: towards endophyte driven farming to improve plant fitness and immunity.World journal of microbiology & biotechnology · 2020Review
- Endophytic Fungi Activated Similar Defense Strategies ofFrontiers in microbiology · 2020Article
- Transcriptome Analysis of Choke Stroma and Asymptomatic Inflorescence Tissues Reveals Changes in Gene Expression in BothMicroorganisms · 2019Article
- An expanded subfamily of G-protein-coupled receptor genes in Fusarium graminearum required for wheat infection.Nature microbiology · 2019Article
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4 authors at 2 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
Epichloë festucae forms a mutualistic symbiotic association with Lolium perenne. This biotrophic fungus systemically colonizes the intercellular spaces of aerial tissues to form an endophytic hyphal network. E. festucae also grows as an epiphyte, but the mechanism for leaf surface colonization is not known. Here we identify an appressorium-like structure, which we call an expressorium that allows endophytic hyphae to penetrate the cuticle from the inside of the leaf to establish an epiphytic hyphal net on the surface of the leaf. We used a combination of scanning electron, transmission electron and confocal laser scanning microscopy to characterize this novel fungal structure and determine the composition of the hyphal cell wall using aniline blue and wheat germ agglutinin labelled with Alexafluor-488. Expressoria differentiate immediately below the cuticle in the leaf blade and leaf sheath intercalary cell division zones where the hyphae grow by tip growth. Differentiation of this structure requires components of both the NoxA and NoxB NADPH oxidase complexes. Major remodelling of the hyphal cell wall occurs following exit from the leaf. These results establish that the symbiotic association of E. festucae with L. perenne involves an interconnected hyphal network of both endophytic and epiphytic hyphae.
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