ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
Isolation and expression of two polyketide synthase genes from Trichoderma harzianum 88 during mycoparasitism.
Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 17 citations in OpenAlex.
- Polyketide synthase gene clusters in the ericoid mycorrhizal fungusFrontiers in plant science · 2026Article
- Strain improvement ofFrontiers in microbiology · 2023Review
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- Complete Genome Sequences and Genome-Wide Characterization ofMicrobiology spectrum · 2021Article
- Molecular methods unravel the biosynthetic potential ofRSC advances · 2021Review
- Effects of ultraviolet irradiation on theHeliyon · 2019Article
- Functional analysis of polyketide synthase genes in the biocontrol fungus Clonostachys rosea.Scientific reports · 2018Article
- Genomic characterization of Trichoderma atrobrunneum (T. harzianum species complex) ITEM 908: insight into the genetic endowment of a multi-target biocontrol strain.BMC genomics · 2018Article
- Volatile Compound-Mediated Recognition and Inhibition BetweenFrontiers in microbiology · 2018Article
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6 authors at 3 institutions in 1 country.
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Abstract
Metabolites of mycoparasitic fungal species such as Trichoderma harzianum 88 have important biological roles. In this study, two new ketoacyl synthase (KS) fragments were isolated from cultured Trichoderma harzianum 88 mycelia using degenerate primers and analysed using a phylogenetic tree. The gene fragments were determined to be present as single copies in Trichoderma harzianum 88 through southern blot analysis using digoxigenin-labelled KS gene fragments as probes. The complete sequence analysis in formation of pksT-1 (5669bp) and pksT-2 (7901bp) suggests that pksT-1 exhibited features of a non-reducing type I fungal PKS, whereas pksT-2 exhibited features of a highly reducing type I fungal PKS. Reverse transcription polymerase chain reaction indicated that the isolated genes are differentially regulated in Trichoderma harzianum 88 during challenge with three fungal plant pathogens, which suggests that they participate in the response of Trichoderma harzianum 88 to fungal plant pathogens. Furthermore, disruption of the pksT-2 encoding ketosynthase-acyltransferase domains through Agrobacterium-mediated gene transformation indicated that pksT-2 is a key factor for conidial pigmentation in Trichoderma harzianum 88.
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