ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
Detection of carboxylesterase and esterase activity in culturable gut bacterial flora isolated from diamondback moth, Plutella xylostella (Linnaeus), from India and its possible role in indoxacarb degradation.
Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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46 citing papers in PubMed, 139 citations in OpenAlex.
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- Isolation and Characterization of Chlorpyrifos-Degrading Gut Bacteria from Field-Collected Larvae ofBiology · 2025Article
- Gut bacteria contribute to fenvalerate resistance in the tomato leafminer, Tuta absoluta.Scientific reports · 2025Article
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- Gut Microbiota MediateInternational journal of molecular sciences · 2024Article
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- The role of insect gut microbiota in host fitness, detoxification and nutrient supplementation.Antonie van Leeuwenhoek · 2024Review
- Bacterial biota associated with the invasive insect pest Tuta absoluta (Meyrick).Scientific reports · 2024Article
- Gut microbiome-derived hydrolases-an underrated target of natural product metabolism.Frontiers in cellular and infection microbiology · 2024Review
- Insect Microbial Symbionts: Ecology, Interactions, and Biological Significance.Microorganisms · 2023Review
- Impact of gut microbiota composition on black cutworm, Agrotis ipsilon (hufnagel) metabolic indices and pesticide degradation.Animal microbiome · 2023Article
- Symbiotic Bacteria ModulateInternational journal of molecular sciences · 2023Article
- Evaluation of Transgenerational Effects of Sublethal Imidacloprid and Diversity of Symbiotic Bacteria onInsects · 2023Article
- The gut symbiont Sphingomonas mediates imidacloprid resistance in the important agricultural insect pest Aphis gossypii Glover.BMC biology · 2023Article
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Authors and funding
5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Diamondback moth (DBM), Plutella xylostella (Linnaeus), is a notorious pest of brassica crops worldwide and is resistant to all groups of insecticides. The insect system harbors diverse groups of microbiota, which in turn helps in enzymatic degradation of xenobiotic-like insecticides. The present study aimed to determine the diversity of gut microflora in DBM, quantify esterase activity and elucidate their possible role in degradation of indoxacarb. We screened 11 geographic populations of DBM in India and analyzed them for bacterial diversity. The culturable gut bacterial flora underwent molecular characterization with 16S rRNA. We obtained 25 bacterial isolates from larvae (n=13) and adults (n=12) of DBM. In larval gut isolates, gammaproteobacteria was the most abundant (76%), followed by bacilli (15.4%). Molecular characterization placed adult gut bacterial strains into three major classes based on abundance: gammaproteobacteria (66%), bacilli (16.7%) and flavobacteria (16.7%). Esterase activity from 19 gut bacterial isolates ranged from 0.072 to 2.32μmol/min/mg protein. Esterase bands were observed in 15 bacterial strains and the banding pattern differed in Bacillus cereus - KC985225 and Pantoea agglomerans - KC985229. The bands were characterized as carboxylesterase with profenofos used as an inhibitor. Minimal media study showed that B. cereus degraded indoxacarb up to 20%, so it could use indoxacarb for metabolism and growth. Furthermore, esterase activity was greater with minimal media than control media: 1.87 versus 0.26μmol/min/mg protein. Apart from the insect esterases, bacterial carboxylesterase may aid in the degradation of insecticides in DBM.
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