Evidence map›Paper›PMID 26991262›Full record

ArticlePediatric research2016

Liganded retinoic acid X receptor α represses connexin 43 through a potential retinoic acid response element in the promoter region.

Ruoyi Gu, Jun Xu, Yixiang Lin, Jing Zhang, Huijun Wang, Wei Sheng, Duan Ma, Xiaojing Ma, Guoying Huang

Open access · bronzeAbstract read
PubMed Publisher
In one paragraph

Article in Pediatric research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Ruoyi GuChildren's Hospital of Fudan University, Shanghai, China.
Jun XuChildren's Hospital of Fudan University, Shanghai, China.
Yixiang LinChildren's Hospital of Fudan University, Shanghai, China.
Jing ZhangChildren's Hospital of Fudan University, Shanghai, China.
Huijun WangChildren's Hospital of Fudan University, Shanghai, China.
Wei ShengChildren's Hospital of Fudan University, Shanghai, China.
Duan MaShanghai Key Laboratory of Birth Defects, Shanghai, China.
Xiaojing MaChildren's Hospital of Fudan University, Shanghai, China.
Guoying HuangChildren's Hospital of Fudan University, Shanghai, China.
Children's Hospital of Fudan University · CNShanghai Medical College of Fudan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionRetinoic acid X receptor alpha (RXRα) and Connexin 43 (Cx43) both play a crucial role in cardiogenesis. However, little is known about the interplay mechanism between the RXRα and Cx43.

methodsThe activations of retinoic acid response element (RARE) in Cx43 were measured by luciferase transfection assay. Electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation (ChIP) was performed to prove that RXRα can directly bind to the RARE sequence. Quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting were used to analyze the RXRα and Cx43 mRNA level and protein level in cells.

resultsIn this study, we confirmed the negative association of the gene expression between the RXRα and Cx43 in the cell level. Interestingly, a functional RARE was detected in the region from -1,426 to -314 base pairs upstream from the transcriptional start site of Cx43. Moreover, we also prove that RXRα can directly bind to this RARE sequence in vitro and in vivo.

conclusionsRXRα negatively regulates the transcription and expression by directly binding to the RARE in the promoter of Cx43. The RARE-like sequence harbored in the Cx43 promoter region may serve as a functional RARE in the retinoic acid (RA) signaling pathway.

Indexed as

Promoter Regions, GeneticAnimalsChromatin ImmunoprecipitationConnexin 43Gene Expression RegulationHeartHeLa CellsHumansMicroscopy, FluorescenceMutagenesis, Site-DirectedReal-Time Polymerase Chain ReactionResponse ElementsRetinoic Acid Receptor alphaSignal TransductionConnexin 43Retinoic Acid Receptor alpha

Identifiers

PMID26991262
OpenAlexW2315204034

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.