ArticleAngewandte Chemie (International ed. in English)2016
Firefly Luciferase Mutants Allow Substrate-Selective Bioluminescence Imaging in the Mouse Brain.
Article in Angewandte Chemie (International ed. in English), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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Who cites it
29 citing papers in PubMed, 60 citations in OpenAlex.
- PEGylated ATP-Independent Luciferins for Noninvasive High-Sensitivity High-Speed Bioluminescence Imaging.ACS chemical biology · 2025Article
- Recent advances in bioluminescent probes for neurobiology.Neurophotonics · 2024Article
- Firefly luciferin methyl ester illuminates the activity of multiple serine hydrolases.Chemical communications (Cambridge, England) · 2023Article
- Biochemical Analysis Leads to Improved Orthogonal Bioluminescent Tools.Chembiochem : a European journal of chemical biology · 2023Article
- A luciferase prosubstrate and a red bioluminescent calcium indicator for imaging neuronal activity in mice.Nature communications · 2022Article
- Modulation of spatiotemporal dynamics in the bromate-sulfite-ferrocyanide reaction system by visible light.RSC advances · 2022Article
- A catalyst-free co-reaction system of long-lasting and intensive chemiluminescence applied to the detection of alkaline phosphatase.Mikrochimica acta · 2022Article
- Brightening up Biology: Advances in Luciferase Systems forACS chemical biology · 2021Review
- Coumarin luciferins and mutant luciferases for robust multi-component bioluminescence imaging.Chemical science · 2021Article
- Multicomponent Bioluminescence Imaging with Naphthylamino Luciferins.Chembiochem : a European journal of chemical biology · 2021Article
- Rapid Multicomponent Bioluminescence ImagingACS chemical biology · 2021Article
- How to Select Firefly Luciferin Analogues for In Vivo Imaging.International journal of molecular sciences · 2021Review
- Visualization of activity-regulated BDNF expression in the living mouse brain using non-invasive near-infrared bioluminescence imaging.Molecular brain · 2020Article
- Multicomponent Bioluminescence Imaging with a π-Extended Luciferin.Journal of the American Chemical Society · 2020Article
- Enzymatic promiscuity and the evolution of bioluminescence.The FEBS journal · 2020Review
- Bioluminescence imaging in mice with synthetic luciferin analogues.Methods in enzymology · 2020Article
- Caged luciferins for bioluminescent activity-based sensing.Current opinion in biotechnology · 2019Review
- Building Biological Flashlights: Orthogonal Luciferases and Luciferins forAccounts of chemical research · 2019Review
- Development and Applications of Bioluminescent and Chemiluminescent Reporters and Biosensors.Annual review of analytical chemistry (Palo Alto, Calif.) · 2019Review
- Computational investigation into the fluorescence of luciferin analogues.Journal of computational chemistry · 2019Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Bioluminescence imaging is a powerful approach for visualizing specific events occurring inside live mice. Animals can be made to glow in response to the expression of a gene, the activity of an enzyme, or the growth of a tumor. But bioluminescence requires the interaction of a luciferase enzyme with a small-molecule luciferin, and its scope has been limited by the mere handful of natural combinations. Herein, we show that mutants of firefly luciferase can discriminate between natural and synthetic substrates in the brains of live mice. When using adeno-associated viral (AAV) vectors to express luciferases in the brain, we found that mutant luciferases that are inactive or weakly active with d-luciferin can light up brightly when treated with the aminoluciferins CycLuc1 and CycLuc2 or their respective FAAH-sensitive luciferin amides. Further development of selective luciferases promises to expand the power of bioluminescence and allow multiple events to be imaged in the same live animal.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.