ArticleMicrobiologyOpen2016
Estimation of Mycobacterium avium subsp. paratuberculosis load in raw bulk tank milk in Emilia-Romagna Region (Italy) by qPCR.
Article in MicrobiologyOpen, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed.
- Candidate Faecal microRNAs as Non-Invasive Biomarkers for Bovine Paratuberculosis inInternational journal of molecular sciences · 2026Article
- Molecular detection and culture ofFrontiers in public health · 2026Article
- VaryingMicrobiology spectrum · 2024Article
- Assessment of virus concentration methods for detecting SARS-CoV-2 IN wastewater.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Real-Time PCR Protocol for Detection and Quantification of Three Pathogenic Members of the Vibrionaceae Family.Microorganisms · 2022Article
- Isothermal inactivation ofFrontiers in microbiology · 2022Article
- Goat Paratuberculosis: Experimental Model for the Evaluation of Mycobacterium Persistence in Raw Milk Cheese.Microorganisms · 2021Article
- Paratuberculosis in Captive Scimitar-Horned Oryxes (Animals : an open access journal from MDPI · 2020Article
- Article
- Estimation of Performance Characteristics of Analytical Methods forFrontiers in microbiology · 2019Article
- A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and Everything.Frontiers in microbiology · 2017Review
- Comparison among the Quantification of Bacterial Pathogens by qPCR, dPCR, and Cultural Methods.Frontiers in microbiology · 2017Article
- The Potential Role of Direct and Indirect Contacts on Infection Spread in Dairy Farm Networks.PLoS computational biology · 2017Article
- Estimation of Mycobacterium avium subsp. paratuberculosis load in raw bulk tank milk in Emilia-Romagna Region (Italy) by qPCR.MicrobiologyOpen · 2016Article
- Improved Culture Medium (TiKa) forFrontiers in microbiology · 2016Article
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Authors and funding
10 authors.
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Abstract
Consumption of milk and dairy products is considered one of the main routes of human exposure to Mycobacterium avium subsp. paratuberculosis (MAP). Quantitative data on MAP load in raw cows' milk are essential starting point for exposure assessment. Our study provides this information on a regional scale, estimating the load of MAP in bulk tank milk (BTM) produced in Emilia-Romagna region (Italy). The survey was carried out on 2934 BTM samples (88.6% of the farms herein present) using two different target sequences for qPCR (f57 and IS900). Data about the performances of both qPCRs are also reported, highlighting the superior sensitivity of IS900-qPCR. Seven hundred and eighty-nine samples tested MAP-positive (apparent prevalence 26.9%) by IS900 qPCR. However, only 90 of these samples were quantifiable by qPCR. The quantifiable samples contained a median load of 32.4 MAP cells mL(-1) (and maximum load of 1424 MAP cells mL(-1) ). This study has shown that a small proportion (3.1%) of BTM samples from Emilia-Romagna region contained MAP in excess of the limit of detection (1.5 × 10(1) MAP cells mL(-1) ), indicating low potential exposure for consumers if the milk subsequently undergoes pasteurization or if it is destined to typical hard cheese production.
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