ArticleJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology2016
Clinical evaluation of a single-reaction real-time RT-PCR for pan-dengue and chikungunya virus detection.
Article in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
- Comprehensive conservation profiling enables a robust multiplex RT-qPCR assay for simultaneous pan-detection and serotype discrimination of dengue virus.Microbiology spectrum · 2026Article
- Development of a Single Thermal Cycling Multiplex RT-qPCR Assay for Rapid Arbovirus Detection.Diagnostics (Basel, Switzerland) · 2026Article
- Risk factors associated with dengue and chikungunya seroprevalence and seroconversion among urban populations in western and coastal Kenya.PLoS neglected tropical diseases · 2025Article
- Mosquito Species Diversity and Circulation of Mosquito-Borne Viruses in Selected Provinces of Central Vietnam.Viruses · 2025Article
- Sticky traps for Aedes aegypti surveillance and targeted vector control in Sincelejo, ColombiaBiomedica : revista del Instituto Nacional de Salud · 2025Article
- Dengue severity by serotype and immune status in 19 years of pediatric clinical studies in Nicaragua.PLoS neglected tropical diseases · 2025Article
- Primary exposure to Zika virus is linked with increased risk of symptomatic dengue virus infection with serotypes 2, 3, and 4, but not 1.Science translational medicine · 2024Article
- Chikungunya virus antepartum transmission and abnormal infant outcomes in a cohort of pregnant women in Nigeria.International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2024Article
- Characterization of Dengue Virus Serotype 2 Cosmopolitan Genotype Circulating in Colombia.The American journal of tropical medicine and hygiene · 2023Article
- Primary exposure to Zika virus increases risk of symptomatic dengue virus infection with serotypes 2, 3, and 4 but not serotype 1.medRxiv : the preprint server for health sciences · 2023Article
- Phylodynamics of dengue virus 2 in Nicaragua leading up to the 2019 epidemic reveals a role for lineage turnover.BMC ecology and evolution · 2023Article
- Article
- Simultaneous detection and differentiation of dengue and chikungunya viruses for commercial utility.Virusdisease · 2023Article
- Recent molecular techniques for the diagnosis of Zika and Chikungunya infections: A systematic review.Heliyon · 2022Article
- Development of a Singleplex Real-Time Reverse Transcriptase PCR Assay for Pan-Dengue Virus Detection and Quantification.Viruses · 2022Article
- Majority of pediatric dengue virus infections in Kenya do not meet 2009 WHO criteria for dengue diagnosis.PLOS global public health · 2022Article
- Dengue and Zika virus infections in children elicit cross-reactive protective and enhancing antibodies that persist long term.Science translational medicine · 2021Article
- A dengue outbreak in a rural community in Northern Coastal Ecuador: An analysis using unmanned aerial vehicle mapping.PLoS neglected tropical diseases · 2021Article
- Evolution and epidemiologic dynamics of dengue virus in Nicaragua during the emergence of chikungunya and Zika viruses.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2021Article
- Boosting can explain patterns of fluctuations of ratios of inapparent to symptomatic dengue virus infections.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
backgroundDengue virus (DENV) and chikungunya virus (CHIKV) now co-circulate throughout tropical regions of the world, with billions of people living at risk of infection. The differentiation of these infections is important for epidemiologic surveillance as well as clinical care, though widely-used molecular diagnostics for DENV and CHIKV require the performance of two to four separate PCR reactions for detection.
objectivesIn the current study, we sought to develop and evaluate a single-reaction, multiplex real-time RT-PCR (rRT-PCR) for the detection and differentiation of DENV and CHIKV (the pan-DENV-CHIKV rRT-PCR). STUDY
designFrom an alignment of all available CHIKV complete genome sequences in GenBank, a new CHIKV rRT-PCR was designed for use in multiplex with a previously described assay for pan-DENV detection. Analytical evaluation was performed in accordance with published recommendations, and the pan-DENV-CHIKV rRT-PCR was clinically compared to reference molecular diagnostics for DENV and CHIKV using 182 serum samples from suspected cases in Managua, Nicaragua.
resultsThe pan-DENV-CHIKV rRT-PCR had a dynamic range extending from 7.0 to 2.0 log10copies/μL for each DENV serotype and CHIKV, and the lower limits of 95% detection were 7.9-37.4copies/μL. The pan-DENV-CHIKV rRT-PCR detected DENV in 81 patients compared to 75 using a reference, hemi-nested DENV RT-PCR, and it demonstrated perfect agreement with a reference CHIKV rRT-PCR (54 positive samples).
conclusionsThe single-reaction, multiplex format of the pan-DENV-CHIKV rRT-PCR, combined with sensitive detection of both viruses, has the potential to improve detection while decreasing testing costs and streamlining molecular workflow.
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