ArticleMolecular microbiology2016
Synergistic interaction of glyceraldehydes-3-phosphate dehydrogenase and ArsJ, a novel organoarsenical efflux permease, confers arsenate resistance.
Article in Molecular microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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42 citing papers in PubMed, 102 citations in OpenAlex.
- Deciphering the genetic background of an industrial 2-ketogluconic acid-producing strain Pseudomonas plecoglossicida JUIM01 using whole-genome sequencing.Biotechnology for biofuels and bioproducts · 2026Article
- Synergistic function of ars genes in arsenic detoxification and biosynthesis of organoarsenicals.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2026Review
- Arsenic efflux mechanisms in ectomycorrhizal mushrooms Hebeloma bulbiferum and Hebeloma sinapizans.Applied microbiology and biotechnology · 2026Article
- Arsenic migration and transformation, greenhouse gas dynamics, and their coupled relationships in paddy fields.Frontiers in microbiology · 2026Review
- Mechanisms of Arsenic Interaction inInternational journal of molecular sciences · 2025Review
- Arsenic biosorption mediated by arsenic-binding proteins QueF and QueE inApplied and environmental microbiology · 2025Article
- A reduction-secretion system contributes to roxarsone (V) degradation and efflux in Brevundimonas sp. M20.BMC microbiology · 2025Article
- Isolation of a potentially arsenic-resistant Halomonas elongata strain (ml10562) from hypersaline systems in the Peruvian Andes, Cusco.PloS one · 2025Article
- Arsenic stress triggers active exudation of arsenic-phytochelatin complexes from Lupinus albus roots.Journal of experimental botany · 2024Article
- Review
- Mechanisms of emerging resistance associated with non-antibiotic antimicrobial agents: a state-of-the-art review.The Journal of antibiotics · 2023Review
- Negative Impacts of Arsenic on Plants and Mitigation Strategies.Plants (Basel, Switzerland) · 2023Review
- Proteomic Analysis of Arsenic Resistance during Cyanide Assimilation byInternational journal of molecular sciences · 2023Article
- The ArsQ permease and transport of the antibiotic arsinothricin.Molecular microbiology · 2023Article
- Genome Sequence ofMicrobiology resource announcements · 2023Article
- AsgeneDB: a curated orthology arsenic metabolism gene database and computational tool for metagenome annotation.NAR genomics and bioinformatics · 2022Article
- Article
- Review
- Molecular characterization of glyceraldehyde-3-phosphate dehydrogenase from Eimeria tenella.Parasitology research · 2022Article
- Characterization of Two Highly Arsenic-Resistant Caulobacteraceae Strains ofInternational journal of molecular sciences · 2022Article
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Abstract
Microbial biotransformations are major contributors to the arsenic biogeocycle. In parallel with transformations of inorganic arsenic, organoarsenicals pathways have recently been recognized as important components of global cycling of arsenic. The well-characterized pathway of resistance to arsenate is reduction coupled to arsenite efflux. Here, we describe a new pathway of arsenate resistance involving biosynthesis and extrusion of an unusual pentavalent organoarsenical. A number of arsenic resistance (ars) operons have two genes of unknown function that are linked in these operons. One, gapdh, encodes the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase. The other, arsJ, encodes a major facilitator superfamily (MFS) protein. The two genes were cloned from the chromosome of Pseudomonas aeruginosa. When expressed together, but not alone, in Escherichia coli, gapdh and arsJ specifically conferred resistance to arsenate and decreased accumulation of As(V). Everted membrane vesicles from cells expressing arsJ accumulated As(V) in the presence of purified GAPDH, D-glceraldehylde 3-phosphate (G3P) and NAD(+) . GAPDH forms the unstable organoarsenical 1-arseno-3-phosphoglycerate (1As3PGA). We propose that ArsJ is an efflux permease that extrudes 1As3PGA from cells, where it rapidly dissociates into As(V) and 3-phosphoglycerate (3PGA), creating a novel pathway of arsenate resistance.
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