ArticleNano letters2016
High-Throughput Mechanobiology Screening Platform Using Micro- and Nanotopography.
Article in Nano letters, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 50 citations in OpenAlex.
- Mechanical regulation of lymphocyte activation and function.Journal of cell science · 2024Review
- T Cell engineering for cancer immunotherapy by manipulating mechanosensitive force-bearing receptors.Frontiers in bioengineering and biotechnology · 2023Review
- Micro-Topographies Induce Epigenetic Reprogramming and Quiescence in Human Mesenchymal Stem Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Immunoengineering strategies to enhance vascularization and tissue regeneration.Advanced drug delivery reviews · 2022Review
- High-Content Screening and Analysis of Stem Cell-Derived Neural Interfaces Using a Combinatorial Nanotechnology and Machine Learning Approach.Research (Washington, D.C.) · 2022Article
- Modification of the surface nanotopography of implant devices: A translational perspective.Materials today. Bio · 2021Review
- Expanding Biomaterial Surface Topographical Design Space through Natural Surface Reproduction.Advanced materials (Deerfield Beach, Fla.) · 2021Article
- Multiwell Combinatorial Hydrogel Array for High-Throughput Analysis of Cell-ECM Interactions.ACS biomaterials science & engineering · 2021Article
- Biphasic response of T cell activation to substrate stiffness.Biomaterials · 2021Article
- High-Throughput Methods in the Discovery and Study of Biomaterials and Materiobiology.Chemical reviews · 2021Review
- The CAR T-Cell Mechanoimmunology at a Glance.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Review
- Getting the big picture of cell-matrix interactions: High-throughput biomaterial platforms and systems-level measurements.Current opinion in solid state & materials science · 2020Article
- Modulating T Cell Activation Using Depth Sensing Topographic Cues.Advanced biosystems · 2020Article
- Neuronal contact guidance and YAP signaling on ultra-small nanogratings.Scientific reports · 2020Article
- Application of FRET Biosensors in Mechanobiology and Mechanopharmacological Screening.Frontiers in bioengineering and biotechnology · 2020Review
- T cell activation and immune synapse organization respond to the microscale mechanics of structured surfaces.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Molecular Tension Probes to Investigate the Mechanopharmacology of Single Cells: A Step toward Personalized Mechanomedicine.Advanced healthcare materials · 2018Article
- Review: Bioengineering strategies to probe T cell mechanobiology.APL bioengineering · 2018Review
- Evaluation of the topographical influence on the cellular behavior of human umbilical vein endothelial cells.Advanced biosystems · 2018Article
- Enhanced activation and expansion of T cells using mechanically soft elastomer fibers.Advanced biosystems · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 2 institutions in 1 country.
Funding
Abstract
We herein demonstrate the first 96-well plate platform to screen effects of micro- and nanotopographies on cell growth and proliferation. Existing high-throughput platforms test a limited number of factors and are not fully compatible with multiple types of testing and assays. This platform is compatible with high-throughput liquid handling, high-resolution imaging, and all multiwell plate-based instrumentation. We use the platform to screen for topographies and drug-topography combinations that have short- and long-term effects on T cell activation and proliferation. We coated nanofabricated "trench-grid" surfaces with anti-CD3 and anti-CD28 antibodies to activate T cells and assayed for interleukin 2 (IL-2) cytokine production. IL-2 secretion was enhanced at 200 nm trench width and >2.3 μm grating pitch; however, the secretion was suppressed at 100 nm width and <0.5 μm pitch. The enhancement on 200 nm grid trench was further amplified with the addition of blebbistatin to reduce contractility. The 200 nm grid pattern was found to triple the number of T cells in long-term expansion, a result with direct clinical applicability in adoptive immunotherapy.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.