ArticleAmerican journal of physical anthropology2016
Successful enrichment and recovery of whole mitochondrial genomes from ancient human dental calculus.
Article in American journal of physical anthropology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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35 citing papers in PubMed, 82 citations in OpenAlex.
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- Host Traits Impact the Outcome of Metagenomic Library Preparation From Dental Calculus Samples Across Diverse Mammals.Molecular ecology resources · 2025Article
- A reanalysis of population dynamics in the Casas Grandes region of Northern Mexico using mitochondrial DNA.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Oral microbial diversity in 18th century African individuals from South Carolina.Communications biology · 2024Article
- Metagenomic and paleopathological analyses of a historic documented collection explore ancient dental calculus as a diagnostic tool.Scientific reports · 2024Article
- The evidential value of dental calculus in the identification process.Scientific reports · 2023Article
- Extraction Protocol for Parallel Analysis of Proteins and DNA from Ancient Teeth and Dental Calculus.Journal of proteome research · 2023Article
- Benchmarking software tools for trimming adapters and merging next-generation sequencing data for ancient DNA.Frontiers in bioinformatics · 2023Article
- Neolithic dental calculi provide evidence for environmental proxies and consumption of wild edible fruits and herbs in central Apennines.Communications biology · 2022Article
- Ecology, Not Host Phylogeny, Shapes the Oral Microbiome in Closely Related Species.Molecular biology and evolution · 2022Article
- Ancient Human Genomes and Environmental DNA from the Cement Attaching 2,000-Year-Old Head Lice Nits.Molecular biology and evolution · 2022Article
- Understanding the microbial biogeography of ancient human dentitions to guide study design and interpretation.FEMS microbes · 2022Article
- Whole-exome sequencing of the mummified remains of Cangrande della Scala (1291-1329 CE) indicates the first known case of late-onset Pompe disease.Scientific reports · 2021Article
- Tracking the transition to agriculture in Southern Europe through ancient DNA analysis of dental calculus.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Origins and Evolution of the Primate Hepatitis B Virus.Frontiers in microbiology · 2021Review
- Urine as a high-quality source of host genomic DNA from wild populations.Molecular ecology resources · 2021Article
- Archaeological mitogenomes illuminate the historical ecology of sea otters (Proceedings. Biological sciences · 2020Article
- Multi-omic detection ofPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
- Investigating Plant Micro-Remains Embedded in Dental Calculus of the Phoenician Inhabitants of Motya (Sicily, Italy).Plants (Basel, Switzerland) · 2020Article
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9 authors at 3 institutions in 2 countries.
Funding
Abstract
objectivesArchaeological dental calculus is a rich source of host-associated biomolecules. Importantly, however, dental calculus is more accurately described as a calcified microbial biofilm than a host tissue. As such, concerns regarding destructive analysis of human remains may not apply as strongly to dental calculus, opening the possibility of obtaining human health and ancestry information from dental calculus in cases where destructive analysis of conventional skeletal remains is not permitted. Here we investigate the preservation of human mitochondrial DNA (mtDNA) in archaeological dental calculus and its potential for full mitochondrial genome (mitogenome) reconstruction in maternal lineage ancestry analysis. MATERIALS AND
methodsExtracted DNA from six individuals at the 700-year-old Norris Farms #36 cemetery in Illinois was enriched for mtDNA using in-solution capture techniques, followed by Illumina high-throughput sequencing.
resultsFull mitogenomes (7-34×) were successfully reconstructed from dental calculus for all six individuals, including three individuals who had previously tested negative for DNA preservation in bone using conventional PCR techniques. Mitochondrial haplogroup assignments were consistent with previously published findings, and additional comparative analysis of paired dental calculus and dentine from two individuals yielded equivalent haplotype results. All dental calculus samples exhibited damage patterns consistent with ancient DNA, and mitochondrial sequences were estimated to be 92-100% endogenous. DNA polymerase choice was found to impact error rates in downstream sequence analysis, but these effects can be mitigated by greater sequencing depth. DISCUSSION: Dental calculus is a viable alternative source of human DNA that can be used to reconstruct full mitogenomes from archaeological remains. Am J Phys Anthropol 160:220-228, 2016. © 2016 The Authors American Journal of Physical Anthropology Published by Wiley Periodicals, Inc.
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