ArticleReports of biochemistry & molecular biology2014
Hemolytic and cytotoxic properties of saponin purified from Holothuria leucospilota sea cucumber.
Article in Reports of biochemistry & molecular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 42 citations in OpenAlex.
- Phytochemical small molecules fromAmerican journal of translational research · 2026Review
- Tea seed saponin‑reduced extract ameliorates palmitic acid‑induced insulin resistance in HepG2 cells.Molecular medicine reports · 2024Article
- Plant-based nanoparticles targeting malaria management.Frontiers in pharmacology · 2024Review
- Pharmacological overview of hederagenin and its derivatives.RSC medicinal chemistry · 2023Review
- Synthesis and Antibacterial Activity of Polyalthic Acid Analogs.Antibiotics (Basel, Switzerland) · 2023Article
- A Bibliometric and In Silico-Based Analysis of Anti-Lung Cancer Compounds from Sea Cucumber.Marine drugs · 2023Review
- Sea Cucumber Saponins Derivatives Alleviate Hepatic Lipid Accumulation Effectively in Fatty Acids-Induced HepG2 Cells and Orotic Acid-Induced Rats.Marine drugs · 2022Article
- MIL-101 (Fe) @Ag Rapid Synergistic Antimicrobial and Biosafety Evaluation of Nanomaterials.Molecules (Basel, Switzerland) · 2022Article
- Evaluation of Antimicrobial and Anticancer Activities of Bouea macrophylla Ethanol Extract.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Evaluation of Antidiabetic Properties of Adenosma Bracteosum Bonati Extracts in Mice with Streptozotocin-Induced Diabetes.Methods in molecular biology (Clifton, N.J.) · 2022Article
- An Insight into Saponins from Quinoa (Molecules (Basel, Switzerland) · 2020Review
- Biotechnological Potential of Bacteria Isolated from the Sea CucumberMarine drugs · 2019Article
- Current knowledge and development of hederagenin as a promising medicinal agent: a comprehensive review.RSC advances · 2018Review
- Medicinal and health benefit effects of functional sea cucumbers.Journal of traditional and complementary medicine · 2018Review
- Function of Thelenota ananas saponin desulfated holothurin A in modulating cholesterol metabolism.Scientific reports · 2018Article
- Structure and hemolytic activity relationships of triterpenoid saponins and sapogenins.Journal of natural medicines · 2017Article
- Transcriptional analysis of VEGF-D and TGFβ genes in MCF7 cells exposed to saponin isolated from Holothuria leucospilota (sea cucumber).Reports of biochemistry & molecular biology · 2015Article
- The Cytotoxicity of Dacarbazine Potentiated by Sea Cucumber Saponin in Resistant B16F10 Melanoma Cells through Apoptosis Induction.Avicenna journal of medical biotechnologyArticle
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHolothuroids (sea cucumbers) are members of the phylum echinodermata, which produce saponins. Saponins exhibit a wide spectrum of pharmacological and biological activities. In this study, we isolated the crude saponins from the body wall of the dominant Iranian species of sea cucumber, Holothuria leucospilota (H. leucospilota). The purpose of this study was to confirm the presence of saponins in the Persian Gulf H. leucospilota and study the hemolytic and cytotoxic activities of these compounds.
methodsThe body wall of sea cucumber was dried and powdered and the crude saponins were isolated using various solvents. The crude saponins were further purified by column chromatography using HP-20 resin. The foam test, Thin Layer Chromatography (TLC), hemolytic assay, and Fourier Transform Infrared Spectroscopy (FTIR) confirmed the presence of saponins. Cytotoxicity was analyzed using a 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay on A549 cells, a human lung cancer cell line.
resultsThe foam test, hemolytic assay, and TLC supported the presence of saponin compounds in the 80% ethanol fraction of H. leucospilota. The infrared (IR) spectrum of the extract showed hydroxyl (-OH), alkyl (C-H), ether (C-O) and ester (-C=O) absorption characteristic of teriterpenoid saponins. The C-O-C absorption indicated glycoside linkages to the sapogenins. The crude saponin extracted from sea cucumber was cytotoxic to A549 cells.
conclusionThe 80% ethanol fraction of saponin isolated from H. leucospilota exhibited hemolytic activity and offers promise as an anti-cancer candidate.
Indexed as
Identifiers
26989736PMC4757088W2338948330What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.