Evidence map›Paper›PMID 26989734›Full record

ArticleReports of biochemistry & molecular biology2014

TLR4 and TLR2 expression in biopsy specimens from antral and corporal stomach zones in Helicobacter pylori infections.

Mohammad Reza Khakzad, Ahmad Saffari, Niloofar Mohamadpour, Mojtaba Sankian, Abdolreza Varasteh, Farhad Salari, Mojtaba Meshkat

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in Reports of biochemistry & molecular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. UEG Week 2019 Poster Presentations.United European gastroenterology journal · 2019
    Article
  3. The Relationship between Toll-like Receptors andJournal of immunology research · 2019
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Mohammad Reza KhakzadDepartment of Immunology, Zakariya Research Center, Mashhad Branch, Islamic Azad University, Mashhad, Iran; Allergy Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Ahmad SaffariInternal Medicine Department, Mashhad Branch, Aria Hospital, Islamic Azad University, Mashhad, Iran.
Niloofar MohamadpourDepartment of Microbiology, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
Mojtaba SankianImmunobiochemistry lab, Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Abdolreza VarastehAllergy Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Farhad SalariImmunobiochemistry lab, Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Mojtaba MeshkatDepartment of Immunology, Zakariya Research Center, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
Mashhad University of Medical Sciences · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundIt is not yet known which types of Toll-like receptors (TLRs) are most effective in Helicobacter pylori (H. pylori) recognition. It is also not known which gastric zones have the most prominent roles in TLR-mediated bacterial recognition. The aim of this work was to analyze the expression of TLR2 and TLR4 in biopsy specimens from H. pylori-infected patients.

methodsThirty-eight patients with gastrointestinal disorders were divided into four groups in this study. The groups were: (A) H. pylori infection and peptic ulcer (n=15), (B) peptic ulcer only (n=5), (C) H. pylori infection only (n=10) and (D) control, with neither H. pylori infection nor peptic ulcer (n=8). Biopsy specimens from sites of redness or atrophic mucosa from gastric antrum and body in patients with gastritis were collected. RNAs from the antrum and body specimens were isolated. TLR2 and TLR4 mRNA expression was assessed by RT-PCR and quantified as densitometric ratios of TLR2 and TLR4/β-actin mRNA.

resultsIn the antral zones of H. pylori-infected patients (Groups A and C) TLR2 and TLR4 expression was significantly greater than in uninfected patients (Groups B and D) regardless of peptic ulcers (p < 0.05). In the gastric body samples TLR2 expression was significantly greater in Group C (H. pylori infection only) than in Group B (peptic ulcer only) and TLR4 expression was significantly greater in group A (H. pylori infection and peptic ulcer) than in Group B (peptic ulcer only) (p < 0.05). No significant differences in expression of TLR4 and TLR2 were observed between samples from the antrum and body in same groups.

conclusionsWe conclude that H. pylori infection leads to significant increase in TLR2 and TLR4 molecules expression in antral region related to the control group. Considering the stimulatory effect of H. pylori on TLRs expression in the gastric tissue, we assume that colonization of H. pylori infection might occurs more in the gastric antral region than in the gastric body.

Indexed as

Helicobacter pyloriPeptic ulcerTLR4; TLR2Toll-like receptors

Identifiers

PMID26989734
PMCPMC4757086
OpenAlexW2341058049

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.