ArticleJournal of nanopharmaceutics and drug delivery2013
Evaluation of Chitosan-Tripolyphosphate Nanoparticles as a p-shRNA Delivery Vector: Formulation, Optimization and Cellular Uptake Study.
Article in Journal of nanopharmaceutics and drug delivery, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Exploring Naturally-Derived Targeted Nano Delivery Therapy for Burn Wound Healing with Special Emphasis on Preclinical Outcomes.Current drug delivery · 2025Review
- Development of chitosan lipid nanoparticles to alleviate the pharmacological activity of piperine in the management of cognitive deficit in diabetic rats.Scientific reports · 2024Article
- Antimicrobial and wound healing potential of naphthoquinones encapsulated in nanochitosan.Frontiers in bioengineering and biotechnology · 2023Article
- Functionalized chitosan nanoparticles for cutaneous delivery of a skin whitening agent: an approach to clinically augment the therapeutic efficacy for melasma treatment.Drug delivery · 2022Article
- The Fluorescent Effect of Withania Somnifera Chitosan Nanocomposite as an Effective Contrast Agent for Cancer Theragnostic: Experimental Study in Vitro.Journal of fluorescence · 2022Article
- Design and Development of Levodopa Loaded Polymeric Nanoparticles for Intranasal Delivery.Pharmaceuticals (Basel, Switzerland) · 2022Article
- Chitosan nanoparticle-mediated effect of antimiRNA-324-5p on decreasing the ovarian cancer cell proliferation by regulation of GLI1 expression.BioImpacts : BI · 2022Article
- Near-infrared light-triggered nano-prodrug for cancer gas therapy.Journal of nanobiotechnology · 2021Review
- Phytochemical-Based Nano-Pharmacotherapeutics for Management of Burn Wound Healing.Gels (Basel, Switzerland) · 2021Review
- miRNA Delivery by Nanosystems: State of the Art and Perspectives.Pharmaceutics · 2021Review
- Encapsulation and controlled release of vitamin C in modified cellulose nanocrystal/chitosan nanocapsules.Current research in food science · 2021Article
- Review
- Toward Highly Efficient Cancer Imaging and Therapy Using the Environment-Friendly Chitosan Nanoparticles and NIR Laser.Biosensors · 2019Article
- Plant protein-based hydrophobic fine and ultrafine carrier particles in drug delivery systems.Critical reviews in biotechnology · 2018Review
- Nanomedicine and advanced technologies for burns: Preventing infection and facilitating wound healing.Advanced drug delivery reviews · 2018Review
- Noble metal nanoparticles in biosensors: recent studies and applications.Nanotechnology reviews · 2017Article
- Smart Nanostructures for Cargo Delivery: Uncaging and Activating by Light.Journal of the American Chemical Society · 2017Review
- Nanocaged platforms: modification, drug delivery and nanotoxicity. Opening synthetic cages to release the tiger.Nanoscale · 2017Review
- Temperature-Responsive Smart Nanocarriers for Delivery Of Therapeutic Agents: Applications and Recent Advances.ACS applied materials & interfaces · 2016Review
- Microfluidic systems for stem cell-based neural tissue engineering.Lab on a chip · 2016Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Polysaccharides (especially chitosan) have recently attracted much attention as gene therapy delivery vehicles for their unique properties such as biocompatibility, biodegradability, low toxicity, and controlled release. Nanoparticles have strong potential as a carrier of plasmid short hairpin RNA (p-shRNA). This study aimed to find the optimum conditions for obtaining Chitosan/triphosphate (TPP)/p-shRNA nanoparticles by the ionic gelation method, and investigating the cellular uptake of the optimized nanoparticles. After applying the central composite design of response surface methodology (RSM), the optimum conditions for preparation of nanoparticles with small size and high loading efficiency were: chitosan/TPP ratio = 10, pH = 5.5 and N/P ratio = 11. The resulting nanoparticles had an average size of 172.8 ± 7 nm and loading efficiency of 71.5 ± 5%. SEM images showed spherical and smooth nanoparticles. The nanoparticles complexed with p-shRNA and may protect it against nuclease digestion. Cytotoxicity studies with HeLa and PC3 human cancer cells demonstrated that chitosan/TPP nanoparticles had low toxicity. Cellular uptake studies using HeLa cells showed that the nanoparticles entered the cells (cellular uptake) and delivered DNA, probably due to their favorable Zeta potential (approximately +28 mV) and small size.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.