Evidence map›Paper›PMID 26989284›Full record

ArticleIranian journal of medical sciences2016

Growth Kinetics, Characterization, and Plasticity of Human Menstrual Blood Stem Cells.

Davood Mehrabani, Roshanak Bahrami Nazarabadi, Maryam Kasraeian, Amin Tamadon, Mehdi Dianatpour, Akbar Vahdati, Shahrokh Zare, Farnaz Ghobadi

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in Iranian journal of medical sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
2.8field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 23 citations in OpenAlex.

  1. Iranian journal of medical sciences · 2026
    Article
  2. Therapeutic effect of menstrual blood stem cells in rats with thin endometrium.Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2023
    Article
  3. Review
  4. Article
  5. Article
  6. Article
  7. Review
  8. Article
  9. Article
  10. Article
  11. Strategies to Optimize Adult Stem Cell Therapy for Tissue Regeneration.International journal of molecular sciences · 2016
    Review
  12. Article
  13. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Davood MehrabaniStem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Roshanak Bahrami NazarabadiStem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Maryam KasraeianMaternal-Fetal Medicine Research Center, Perinatology Ward, Department of Gynecology and Obstetrics, Shiraz University of Medical Sciences, Shiraz, Iran.
Amin TamadonStem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Mehdi DianatpourStem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Akbar VahdatiDepartment of Biology, Science and Research Branch, Islamic Azad University, Shiraz, Iran.
Shahrokh ZareStem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Farnaz GhobadiStem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Shiraz University of Medical Sciences · IRStem Cell Technology Research Center · IRIslamic Azad University, Science and Research Branch · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

One of the readily available sources of mesenchymal stem cells (MSCs) is menstrual blood-derived stem cells (Men-SCs), which exhibit characteristics similar to other types of MSCs. This study was performed to determine the growth kinetics, plasticity, and characterization of Men-SCs in women. During spring 2014 in the southern Iranian city of Shiraz, menstrual blood (5 mL) was obtained from 10 women on their third day of menstruation in 2 age groups of 30 to 40 and 40 to 50 years old. Ficoll was used to separate the mononuclear cell fraction. After the Men-SCs were cultured, they were subcultured up to passage 4. Growth behavior and population doubling time were evaluated by seeding 5×10(4) cells into 12- and 24-well culture plates, and the colonies were enumerated. The expression of CD44, CD90, and CD34 was evaluated. The osteogenic potential was assessed by alizarin red staining. The Men-SCs were shown to be plastic adherent and spindle-shaped. Regarding the growth curves in the 12- and 24-well culture plates, it was demonstrated that in the women aged between 30 and 40 years, population doubling time was 55.5 and 62 hours, respectively, while these values in the women aged between 40 and 50 years were 70.4 and 72.4 hours, correspondingly. Positive expression of CD44 and CD90 and negative expression of CD34 were noted. In the osteogenic differentiation medium, the cells differentiated toward osteoblasts. As human Men-SCs are easily collectable without any invasive procedure and are a safe and rapid source of MSCs, they can be a good candidate for stem cell banking and cell transplantation in women.

Indexed as

Menstrual bloodMesenchymal stem cellsPlasticityReverse transcriptase polymerase chain reaction

Identifiers

PMID26989284
PMCPMC4764963
OpenAlexW2282524911

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.