ArticleIranian journal of medical sciences2016
Biphasic Response to Luteolin in MG-63 Osteoblast-Like Cells under High Glucose-Induced Oxidative Stress.
Article in Iranian journal of medical sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 33 citations in OpenAlex.
- The most important medicinal plants affecting sperm and testosterone production: a systematic review.JBRA assisted reproduction · 2022Pooled it
- Therapeutic Potential of Luteolin for Diabetes Mellitus and Its Complications.Chinese journal of integrative medicine · 2025Review
- Evaluation of skin cancer prevention properties of probiotics.Genes & nutrition · 2025Review
- Luteolin's Potential in Managing Osteoporosis and Bone Metabolism Disorders: Preclinical Insights.Drug design, development and therapy · 2025Review
- Effect of luteolin on oxidative stress and inflammation in the human osteoblast cell line hFOB1.19 in an inflammatory microenvironment.BMC pharmacology & toxicology · 2024Article
- Luteolin and Vernodalol as Bioactive Compounds of Leaf and RootPharmaceutics · 2023Article
- Moringa oleifera leaf extract induces osteogenic-like differentiation of human osteosarcoma SaOS2 cells.Journal of traditional and complementary medicine · 2022Article
- Biosynthesis of titanium dioxide nanoparticles using Hypericum perforatum and Origanum vulgare extracts and their main components, hypericin and carvacrol as promising antibacterial agents.Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan · 2022Article
- Emerging Natural-Product-Based Treatments for the Management of Osteoarthritis.Antioxidants (Basel, Switzerland) · 2021Review
- Anacardic acid-mediated regulation of osteoblast differentiation involves mitigation of inflammasome activation pathways.Molecular and cellular biochemistry · 2021Article
- Flavonoids: Classification, Function, and Molecular Mechanisms Involved in Bone Remodelling.Frontiers in endocrinology · 2021Review
- Healthy Effects of Plant Polyphenols: Molecular Mechanisms.International journal of molecular sciences · 2020Review
- Tamarind: A diet-based strategy against lifestyle maladies.Food science & nutrition · 2019Review
- Luteolin supports osteogenic differentiation of human periodontal ligament cells.BMC oral health · 2019Article
- The effects of mumie extract on cell proliferation and enzyme expression of human osteoblast-like cells (MG63).Journal of stem cells & regenerative medicine · 2019Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundClinical evidence indicates the diabetes-induced impairment of osteogenesis caused by a decrease in osteoblast activity. Flavonoids can increase the differentiation and mineralization of osteoblasts in a high-glucose state. However, some flavonoids such as luteolin may have the potential to induce cytotoxicity in osteoblast-like cells. This study was performed to investigate whether a cytoprotective concentration range of luteolin could be separated from a cytotoxic concentration range in human MG-63 osteoblast-like cells in high-glucose condition.
methodsCells were cultured in a normal- or high-glucose medium. Cell viability was determined with the MTT assay. The formation of intracellular reactive oxygen species (ROS) was measured using probe 2',7' -dichlorofluorescein diacetate, and osteogenic differentiation was evaluated with an alkaline phosphatase bioassay.
resultsROS generation, reduction in alkaline phosphatase activity, and cell death induced by high glucose were inhibited by lower concentrations of luteolin (EC50, 1.29±0.23 µM). Oxidative stress mediated by high glucose was also overcome by N-acetyl-L-cysteine. At high concentrations, luteolin caused osteoblast cell death in normal- and high-glucose states (IC50, 34±2.33 and 27±2.42 µM, respectively), as represented by increased ROS and decreased alkaline phosphatase activity.
conclusionOur results indicated that the cytoprotective action of luteolin in glucotoxic condition was manifested in much lower concentrations, by a factor of approximately 26 and 20, than was its cytotoxic activity, which occurred under normal or glucotoxic condition, respectively.
Indexed as
Identifiers
26989282PMC4764961W2260925493What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.