ArticleMicrobial cell factories2016
Medium chain length polyhydroxyalkanoates consisting primarily of unsaturated 3-hydroxy-5-cis-dodecanoate synthesized by newly isolated bacteria using crude glycerol.
Article in Microbial cell factories, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 58 citations in OpenAlex.
- Sulfur Vulcanization and Material Properties of Polyhydroxyalkanoates with Unsaturated Side Chain.Polymers · 2025Article
- Experimental and Computational Insights into Polyurethane Plastic Waste Conversion to Microbial Bioplastic.Current microbiology · 2025Article
- A sustainable synthesis of polyhydroxyalkanoate from stubble waste as a carbon source usingFrontiers in bioengineering and biotechnology · 2024Article
- Impact of phosphorus limitation on medium-chain-length polyhydroxyalkanoate production by activated sludge.Applied microbiology and biotechnology · 2023Article
- Synthetic microbes and biocatalyst designs in Thailand.Biotechnology notes (Amsterdam, Netherlands) · 2023Review
- Bio-conversion of organic wastes towards polyhydroxyalkanoates.Biotechnology notes (Amsterdam, Netherlands) · 2023Review
- Article
- Polyhydroxyalkanoate (PHA) Biopolymer Synthesis by Marine Bacteria of the Malaysian Coral Triangle Region and Mining for PHA Synthase Genes.Microorganisms · 2022Article
- What Is New in the Field of Industrial Wastes Conversion into Polyhydroxyalkanoates by Bacteria?Polymers · 2021Review
- Haloarchaea as Cell Factories to Produce Bioplastics.Marine drugs · 2021Review
- Enhancement of polyhydroxyalkanoate production by co-feeding lignin derivatives with glycerol in Pseudomonas putida KT2440.Biotechnology for biofuels · 2021Article
- Fed-BatchFrontiers in bioengineering and biotechnology · 2021Article
- Polyhydroxybutyrate (PHB) Production Using an Arabinose-Inducible Expression System in Comparison With Cold Shock Inducible Expression System inFrontiers in bioengineering and biotechnology · 2021Article
- New insight into poly (3-hydroxybutyrate) production by Azomonas macrocytogenes isolate KC685000: large scale production, kinetic modeling, recovery and characterization.Molecular biology reports · 2019Article
- Production of a novel medium chain length poly(3-hydroxyalkanoate) using unprocessed biodiesel waste and its evaluation as a tissue engineering scaffold.Microbial biotechnology · 2017Article
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4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundOur study aimed to search for novel bacteria capable of producing polyhydroxyalkanoates (PHAs) using crude glycerol residue obtained from biodiesel production in which used cooking oils were the substrates.
resultsNewly isolated bacteria from soils in Thailand were screened for the efficient production of PHAs from crude glycerol. The bacterial strains were cultivated on glucose, refined glycerol, crude glycerol, or various cooking oils (canola oil, palm oil, soybean oil, sunflower oil, corn oil, grape seed oil, olive oil, rice bran oil, camellia seed oil) for growth and PHA production. The effects of the total organic carbon (TOC) concentration and the mole ratio of carbon to nitrogen were investigated in batch cultivation. (1)H NMR, two dimensional-(1)H-correlation spectroscopy (2D-(1)H-COSY) and (13)C NMR analyses confirmed four bacterial strains were capable of producing medium-chain-length PHAs (mcl-PHAs), consisting of 3-hydroxyoctanoate (3HO) and 3-hydroxy-5-cis-dodecanoate (3H5DD), from crude glycerol. On the basis of phenotypic features and genotypic investigations, the bacterial strains were assigned as: ASC1, Acinetobacter genus (94.9% similarity); ASC2, Pseudomonas genus (99.2% similarity); ASC3, Enterobacter genus (99.2% similarity); ASC4, Bacillus genus (98.4% similarity). The highest amount of mcl-PHAs, 17.5 ± 0.8 g/L (content 61.8 ± 3.3% wt), with 3HO (14.7 ± 2.2 mol %), 3H5DD (85.3 ± 2.2 mol%), and a total biomass of 32.3 ± 0.3 g/L, was obtained from Pseudomonas sp. ASC2 in batch cultivation after 36 h. The mcl-PHAs recovered had a number-average molecular weight (M N) of 3.6 × 10(4) Da. Homopolymeric 3H5DD was obtained when the cultivation time was prolonged to 96 h.
conclusionsNovel PHA-producing strains were isolated and identified. These bacterial strains are able to produce mcl-PHAs from crude glycerol. The mcl-PHAs produced contained a high percentage of 3H5DD, which suggests their future application as softeners mixed with other biomaterials. The unsaturated side chain of 3H5DD monomers containing double bounds offers additional potential for improving the properties of the mcl-PHAs or extending their applications to the food industry.
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