ArticleScientific reports2016
Restoration of normal embryogenesis by mitochondrial supplementation in pig oocytes exhibiting mitochondrial DNA deficiency.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 83 citations in OpenAlex.
- Adipose Stem Cell Mitochondrial Transplantation in ART: From Biological Rationale to Clinical Milestone.Cells · 2026Review
- Nuclear Transfer Perturbs Genomic Balance.Epigenomes · 2026Article
- The need to understand the underlying mechanisms associated with mitochondrial therapies in assisted reproduction before further clinical trials are performed.Human reproduction (Oxford, England) · 2026Review
- Programmed mitophagy at the oocyte-to-zygote transition promotes lineage endurance.Nature cell biology · 2026Article
- The nuclear and mitochondrial genomes need to tango. How is their dance synchronized during development?NAR molecular medicine · 2026Article
- Screening and functional verification of potential metabolites for high fertility in sows.Frontiers in nutrition · 2026Article
- Can the Supplementation of Oocytes with Extra Copies of mtDNA Impact Development Without Being Transmitted? A Molecular Account.International journal of molecular sciences · 2025Article
- Human Placental Stem Cells Derived Exosomes Xenograft Recover Ovarian Function in Training-induced Premature Ovarian Insufficiency Rats.Current stem cell research & therapy · 2025Article
- Supplementation of Oocytes by Microinjection with Extra Copies of mtDNA Alters Metabolite Profiles and Interactions with Expressed Genes in a Tissue-Specific Manner.Biomolecules · 2024Article
- Ovarian aging: energy metabolism of oocytes.Journal of ovarian research · 2024Review
- The role of mtDNA in oocyte quality and embryo development.Molecular reproduction and development · 2023Review
- Mitochondrial DNA Supplementation of Oocytes Has Downstream Effects on the Transcriptional Profiles ofInternational journal of molecular sciences · 2023Article
- Mitochondrial epigenetic modifications and nuclear-mitochondrial communication: A new dimension towards understanding and attenuating the pathogenesis in women with PCOS.Reviews in endocrine & metabolic disorders · 2023Review
- Does supplementation of oocytes with additional mtDNA influence developmental outcome?iScience · 2023Article
- Mitochondrial DNA Deficiency and Supplementation inInternational journal of molecular sciences · 2023Article
- Mitochondria Transfer from Adipose Stem Cells Improves the Developmental Potential of Cryopreserved Oocytes.Biomolecules · 2022Article
- The Molecular Quality and Mitochondrial Activity of Porcine Cumulus-Oocyte Complexes Are Affected by Their Exposure to Three Endocrine-Active Compounds under 3D In Vitro Maturation Conditions.International journal of molecular sciences · 2022Article
- Mitochondrial supplementation of Sus scrofa metaphase II oocytes alters DNA methylation and gene expression profiles of blastocysts.Epigenetics & chromatin · 2022Article
- Article
- Low guanine content and biased nucleotide distribution in vertebrate mtDNA can cause overestimation of non-CpG methylation.NAR genomics and bioinformatics · 2022Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
Abstract
An increasing number of women fail to achieve pregnancy due to either failed fertilization or embryo arrest during preimplantation development. This often results from decreased oocyte quality. Indeed, reduced mitochondrial DNA copy number (mitochondrial DNA deficiency) may disrupt oocyte quality in some women. To overcome mitochondrial DNA deficiency, whilst maintaining genetic identity, we supplemented pig oocytes selected for mitochondrial DNA deficiency, reduced cytoplasmic maturation and lower developmental competence, with autologous populations of mitochondrial isolate at fertilization. Supplementation increased development to blastocyst, the final stage of preimplantation development, and promoted mitochondrial DNA replication prior to embryonic genome activation in mitochondrial DNA deficient oocytes but not in oocytes with normal levels of mitochondrial DNA. Blastocysts exhibited transcriptome profiles more closely resembling those of blastocysts from developmentally competent oocytes. Furthermore, mitochondrial supplementation reduced gene expression patterns associated with metabolic disorders that were identified in blastocysts from mitochondrial DNA deficient oocytes. These results demonstrate the importance of the oocyte's mitochondrial DNA investment in fertilization outcome and subsequent embryo development to mitochondrial DNA deficient oocytes.
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