ArticleScientific reports2016
Electrosensitization assists cell ablation by nanosecond pulsed electric field in 3D cultures.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Inactivation of bacteria using synergistic hydrogen peroxide with split-dose nanosecond pulsed electric field exposures.PloS one · 2024Article
- Inactivation of antibiotic-resistant bacteriaFrontiers in microbiology · 2024Article
- In Vitro Imaging and Molecular Characterization of CaInternational journal of molecular sciences · 2023Article
- Exposure of the SH-SY5Y Human Neuroblastoma Cells to 50-Hz Magnetic Field: Comparison Between Two-Dimensional (2D) and Three-Dimensional (3D) In Vitro Cultures.Molecular neurobiology · 2021Article
- Electroporation safety factor of 300 nanosecond and 10 millisecond defibrillation in Langendorff-perfused rabbit hearts.PloS one · 2021Article
- Article
- Selective distant electrostimulation by synchronized bipolar nanosecond pulses.Scientific reports · 2019Article
- Nanosecond range electric pulse application as a non-viral gene delivery method: proof of concept.Scientific reports · 2018Article
- Membrane Permeabilization of Pathogenic Yeast in Alternating Sub-microsecond Electromagnetic Fields in Combination with Conventional Electroporation.The Journal of membrane biology · 2018Article
- Moderate Heat Application Enhances the Efficacy of Nanosecond Pulse Stimulation for the Treatment of Squamous Cell Carcinoma.Technology in cancer research & treatment · 2018Article
- Electrosensitization Increases Antitumor Effectiveness of Nanosecond Pulsed Electric FieldsTechnology in cancer research & treatment · 2017Article
- Activation of the phospholipid scramblase TMEM16F by nanosecond pulsed electric fields (nsPEF) facilitates its diverse cytophysiological effects.The Journal of biological chemistry · 2017Article
- Synergistic combinations of short high-voltage pulses and long low-voltage pulses enhance irreversible electroporation efficacy.Scientific reports · 2017Article
- Controllable Moderate Heating Enhances the Therapeutic Efficacy of Irreversible Electroporation for Pancreatic Cancer.Scientific reports · 2017Article
- Delayed hypersensitivity to nanosecond pulsed electric field in electroporated cells.Scientific reports · 2017Article
- Selective susceptibility to nanosecond pulsed electric field (nsPEF) across different human cell types.Cellular and molecular life sciences : CMLS · 2017Article
- Irreversible electroporation ablation area enhanced by synergistic high- and low-voltage pulses.PloS one · 2017Article
- Pulsed Electromagnetic Field Assisted in vitro Electroporation: A Pilot Study.Scientific reports · 2016Article
- Article
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Authors and funding
4 authors.
Funding
Abstract
Previous studies reported a delayed increase of sensitivity to electroporation (termed "electrosensitization") in mammalian cells that had been subjected to electroporation. Electrosensitization facilitated membrane permeabilization and reduced survival in cell suspensions when the electric pulse treatments were split in fractions. The present study was aimed to visualize the effect of sensitization and establish its utility for cell ablation. We used KLN 205 squamous carcinoma cells embedded in an agarose gel and cell spheroids in Matrigel. A local ablation was created by a train of 200 to 600 of 300-ns pulses (50 Hz, 300-600 V) delivered by a two-needle probe with 1-mm inter-electrode distance. In order to facilitate ablation by engaging electrosensitization, the train was split in two identical fractions applied with a 2- to 480-s interval. At 400-600 V (2.9-4.3 kV/cm), the split-dose treatments increased the ablation volume and cell death up to 2-3-fold compared to single-train treatments. Under the conditions tested, the maximum enhancement of ablation was achieved when two fractions were separated by 100 s. The results suggest that engaging electrosensitization may assist in vivo cancer ablation by reducing the voltage or number of pulses required, or by enabling larger inter-electrode distances without losing the ablation efficiency.
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