ArticleJournal of the American Chemical Society2016
A Chemical Controller of SNARE-Driven Membrane Fusion That Primes Vesicles for Ca(2+)-Triggered Millisecond Exocytosis.
Article in Journal of the American Chemical Society, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Lithium fine tunes lipid membranes through phospholipid binding.Scientific reports · 2025Article
- Apolipoproteins L1 and L3 control mitochondrial membrane dynamics.Cell reports · 2023Article
- The Get1/2 insertase forms a channel to mediate the insertion of tail-anchored proteins into the ER.Cell reports · 2023Article
- Construction and validation of a risk model based on the key SNARE proteins to predict the prognosis and immune microenvironment of gliomas.Frontiers in molecular neuroscience · 2023Article
- SNARE Modulators and SNARE Mimetic Peptides.Biomolecules · 2022Review
- The beginning and the end of SNARE-induced membrane fusion.FEBS open bio · 2022Review
- Nascent fusion pore opening monitored at single-SNAREpin resolution.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Reduction of focal sweating by lipid nanoparticle-delivered myricetin.Scientific reports · 2020Article
- Characterization ofMolecules (Basel, Switzerland) · 2020Article
- Search for a minimal machinery for CaNeuroscience · 2019Article
- Simulations Reveal Multiple Intermediates in the Unzipping Mechanism of Neuronal SNARE Complex.Biophysical journal · 2018Article
- SolubleFrontiers in immunology · 2018Article
- Spatially encoded fast single-molecule fluorescence spectroscopy with full field-of-view.Scientific reports · 2017Article
- Energetics, kinetics, and pathway of SNARE folding and assembly revealed by optical tweezers.Protein science : a publication of the Protein Society · 2017Review
- Visualization of SNARE-Mediated Hemifusion between Giant Unilamellar Vesicles Arrested by Myricetin.Frontiers in molecular neuroscience · 2017Article
- Hemifusion in Synaptic Vesicle Cycle.Frontiers in molecular neuroscience · 2017Review
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Authors and funding
11 authors.
Funding
Abstract
Membrane fusion is mediated by the SNARE complex which is formed through a zippering process. Here, we developed a chemical controller for the progress of membrane fusion. A hemifusion state was arrested by a polyphenol myricetin which binds to the SNARE complex. The arrest of membrane fusion was rescued by an enzyme laccase that removes myricetin from the SNARE complex. The rescued hemifusion state was metastable and long-lived with a decay constant of 39 min. This membrane fusion controller was applied to delineate how Ca(2+) stimulates fusion-pore formation in a millisecond time scale. We found, using a single-vesicle fusion assay, that such myricetin-primed vesicles with synaptotagmin 1 respond synchronously to physiological concentrations of Ca(2+). When 10 μM Ca(2+) was added to the hemifused vesicles, the majority of vesicles rapidly advanced to fusion pores with a time constant of 16.2 ms. Thus, the results demonstrate that a minimal exocytotic membrane fusion machinery composed of SNAREs and synaptotagmin 1 is capable of driving membrane fusion in a millisecond time scale when a proper vesicle priming is established. The chemical controller of SNARE-driven membrane fusion should serve as a versatile tool for investigating the differential roles of various synaptic proteins in discrete fusion steps.
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