ArticleJournal of oral microbiology2016
Comparative analysis of bacterial profiles in unstimulated and stimulated saliva samples.
Article in Journal of oral microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03225950 (Interaction Between Immune Cells and Bacteria Associated With Periodontitis), which is not on this map. Cited by 50 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Interaction Between Immune Cells and Bacteria Associated With Periodontitis
Who cites it
50 citing papers in PubMed, 1 synthesis or guideline pooled it, 73 citations in OpenAlex.
- Assessment of salivary matrix metalloproteinase (MMP8) and activated salivary matrix metalloproteinase (aMMP8) in periodontitis patients: a systematic review and meta-analysis.Frontiers in oral health · 2025Pooled it
- Cranberry Polyphenol Extract (CPE) Oral Rinse Improves Salivary Microbiome in 6-n-Propylthiouracil (PROP) Non-Tasters and Palatability of Aronia Juice.International journal of molecular sciences · 2026Article
- Oral Microbiome in Oral Cancer Research from Sampling to Analysis: Strategies, Challenges, and Recommendations.Cancers · 2025Review
- Exploring Relationships Within the Microbiome of Root Canal Infections and the Influence of Associated Clinical Parameters.International endodontic journal · 2025Article
- Differential microbiome response to sucrose in caries-free and caries-affected individuals.AMB Express · 2025Article
- Association of Corticosteroid Inhaler Type with Saliva Microbiome in Moderate-to-Severe Pediatric Asthma.Biomedicines · 2025Article
- Comparative evaluation of antimicrobial toothpastes on periodontal bacteria in orthodontic patients: A randomized controlled study.Journal of orthodontic science · 2025Article
- Salivary microbiome in peritoneal dialysis patients with and without sarcopenia: A pilot study.PloS one · 2025Article
- Oral microbiome as a diagnostic biomarker for pancreatic cancer: a systematic review and meta-analysis of diagnostic accuracy.Journal of oral microbiology · 2025Review
- Sampling strategies for digestive system flora studies: current research and perspectives.PeerJ · 2025Review
- Sample Preparation for Metabolomic Analysis in Exercise Physiology.Biomolecules · 2024Review
- Stimulation of Saliva Affects the Release of Aroma in Wine: A Study of Microbiota, Biochemistry, and Participant Origin.Journal of agricultural and food chemistry · 2024Article
- Liquid chromatography-tandem mass spectrometry method for mycophenolic acid and its glucuronide determination in saliva samples from children with nephrotic syndrome.Pharmacological reports : PR · 2024Article
- Saliva sampling method influences oral microbiome composition and taxa distribution associated with oral diseases.PloS one · 2024Article
- Saliva Collection Methods Among Children and Adolescents: A Scoping Review.Molecular diagnosis & therapy · 2024Article
- The Impact of Competitive Sports on Oral Health: Exploring Their Relationship with Salivary Oxidative Stress in Children.Healthcare (Basel, Switzerland) · 2023Article
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- The Influence of Periodontal Diseases and the Stimulation of Saliva Secretion on the Course of the Acute Phase of Ischemic Stroke.Journal of clinical medicine · 2022Article
- Saliva sampling methods. Cariogenic streptococci count using two different methods of saliva collection in children.Acta odontologica latinoamericana : AOL · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
objectiveThe microbial profiles of stimulated saliva samples have been shown to differentiate between patients with periodontitis, patients with dental caries, and orally healthy individuals. Saliva was stimulated to allow for easy and rapid collection; however, microbial composition may not reflect the more natural, unstimulated state. The purpose of this study was to validate whether stimulated saliva is an adequate surrogate for unstimulated saliva in determining salivary microbiomes.
designUnstimulated (n=20) and stimulated (n=20) saliva samples were collected from 20 orally and systemically healthy, non-smoking participants. Salivary bacterial profiles were analyzed by means of the Human Oral Microbe Identification using Next Generation Sequencing (HOMINGS), and statistical analysis was performed using Mann-Whitney test with Benjamini-Hochberg's correction for multiple comparison, cluster analysis, principal component analysis, and correspondence analysis.
resultsFrom a total of 40 saliva samples, 496 probe targets were identified with a mean number of targets per sample of 203 (range: 146-303), and a mean number of probe targets of 206 and 200 in unstimulated and stimulated saliva samples, respectively (p=0.62). Based on all statistical methods used for this study, the microbial profiles of unstimulated and stimulated saliva samples collected from the same person were not statistically significantly different.
conclusionsAnalysis of bacterial salivary profiles in unstimulated and stimulated saliva samples collected from the same individual showed comparable results. Thus, the results verify that stimulated saliva is an adequate surrogate of unstimulated saliva for microbiome-related studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.