ArticlePloS one2016
The Relevance of a Novel Quantitative Assay to Detect up to 40 Major Streptococcus pneumoniae Serotypes Directly in Clinical Nasopharyngeal and Blood Specimens.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Characteristics of Streptococcus pneumoniae colonising Cypriot children during a period of high PCV10 and PCV13 vaccine coverage: serotypes and antimicrobial resistance.BMC infectious diseases · 2026Article
- Nasopharyngeal Carriage, Antimicrobial Resistance, and Serotype Distribution ofAntibiotics (Basel, Switzerland) · 2025Article
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- Prevalence, Clinical Severity, and Serotype Distribution of Pneumococcal Pneumonia Among Adults Hospitalized With Community-Acquired Pneumonia in Tennessee and Georgia, 2018-2022.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2024Article
- Epitope-level profiling in children with mitochondrial disease reveals limitations in the antibacterial antibody repertoire.Molecular genetics and metabolism · 2023Article
- Bacterial and viral etiology of acute respiratory infection among the Forcibly Displaced Myanmar Nationals (FDMNs) in fragile settings in Cox's Bazar- a prospective case-control study.PLoS neglected tropical diseases · 2023Article
- Optimization of a high-throughput nanofluidic real-time PCR to detect and quantify of 15 bacterial species and 92 Streptococcus pneumoniae serotypes.Scientific reports · 2023Article
- Epidemiology and serotype distribution ofFrontiers in public health · 2023Article
- Co-infection with dual Streptococcus pneumoniae serotypes as a cause of pediatric bacterial meningitis in Iran: a multi-center cross-sectional study.BMC infectious diseases · 2022Article
- Modeling of pneumococcal serogroup 10 capsular polysaccharide molecular conformations provides insight into epitopes and observed cross-reactivity.Frontiers in molecular biosciences · 2022Article
- PCR and Culture Analysis ofMicroorganisms · 2021Article
- Article
- MALDI-TOF mass spectrometry for sub-typing of Streptococcus pneumoniae.BMC microbiology · 2020Article
- PMParticle and fibre toxicology · 2020Article
- Molecular serotype-specific identification of Streptococcus pneumoniae using loop-mediated isothermal amplification.Scientific reports · 2019Article
- Article
- Pneumococcal Community-Acquired Pneumonia Detected by Serotype-Specific Urinary Antigen Detection Assays.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2018Article
- Serotype Distribution of ClinicalThe American journal of tropical medicine and hygiene · 2018Article
- Investigating Pneumonia Etiology Among Refugees and the Lebanese population (PEARL): A study protocol.Gates open research · 2018Article
- Loop-Mediated Isothermal Amplification Methods for Diagnosis of Bacterial Meningitis.Frontiers in pediatrics · 2018Review
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For epidemiological and surveillance purposes, it is relevant to monitor the distribution and dynamics of Streptococcus pneumoniae serotypes. Conventional serotyping methods do not provide rapid or quantitative information on serotype loads. Quantitative serotyping may enable prediction of the invasiveness of a specific serotype compared to other serotypes carried. Here, we describe a novel, rapid multiplex real-time PCR assay for identification and quantification of the 40 most prevalent pneumococcal serotypes and the assay impacts in pneumonia specimens from emerging and developing countries. Eleven multiplex PCR to detect 40 serotypes or serogroups were optimized. Quantification was enabled by reference to standard dilutions of known bacterial load. Performance of the assay was evaluated to specifically type and quantify S. pneumoniae in nasopharyngeal and blood samples from adult and pediatric patients hospitalized with pneumonia (n = 664) from five different countries. Serogroup 6 was widely represented in nasopharyngeal specimens from all five cohorts. The most frequent serotypes in the French, South African, and Brazilian cohorts were 1 and 7A/F, 3 and 19F, and 14, respectively. When both samples were available, the serotype in blood was always present as carriage with other serotypes in the nasopharynx. Moreover, the ability of a serotype to invade the bloodstream may be linked to its nasopharyngeal load. The mean nasopharyngeal concentration of the serotypes that moved to the blood was 3 log-fold higher than the ones only found in the nasopharynx. This novel, rapid, quantitative assay may potentially predict some of the S. pneumoniae serotypes invasiveness and assessment of pneumococcal serotype distribution.
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