ArticlePloS one2016
Oral Samples as Non-Invasive Proxies for Assessing the Composition of the Rumen Microbial Community.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
59 citing papers in PubMed, 129 citations in OpenAlex.
- Methane emissions in beef cattle: A review of measurements and mitigation strategies.Veterinary and animal science · 2026Review
- Lactiplantibacillus plantarum and Pediococcus acidilactici strains isolated from cattle gut show probiotic potential.BMC microbiology · 2026Article
- Longitudinal Change in Rumen-Associated Bacterial Communities Following Aso Limonite Supplementation in Japanese Brown Cattle.Animals : an open access journal from MDPI · 2026Article
- Microbial landscapes in dairy cow diseases: from localized dysbiosis to inter-organ axes.NPJ biofilms and microbiomes · 2026Review
- Short communication: oral microbiome as a potential proxy for methane emissions in grazing tropical composite beef cattle.Journal of animal science · 2026Article
- Metagenomic Applications to Herbivore Gut Microbiomes: A Comprehensive Review of Microbial Diversity and Host Interactions.Animals : an open access journal from MDPI · 2025Review
- Evaluation of esophageal tubing and buccal swabbing versus rumen cannula to characterize ruminal microbiota in cows fed contrasting diets.Scientific reports · 2025Article
- Correlating the oral swab microbial community with milk production metrics in Holstein dairy cows.mSphere · 2025Article
- Gut Microbiota of Ruminants and Monogastric Livestock: An Overview.Animals : an open access journal from MDPI · 2025Review
- Ruminal-buccal microbiota transmission and their diagnostic roles in subacute rumen acidosis in dairy goats.Journal of animal science and biotechnology · 2025Article
- Investigating the establishment of the rumen and oral bacterial communities in beef cattle and assessing the applicability of using the oral bacterial community composition as a proxy for rumen bacterial community structure in cattle.Frontiers in microbiology · 2025Article
- Temporal establishment of the colon microbiota in Angus calves from birth to post-weaning.PloS one · 2025Article
- Complex Interactions between the Human Major Histocompatibility Complex (MHC) and Microbiota: Their Roles in Disease Pathogenesis and Immune System Regulation.Biomedicines · 2024Review
- Rumen protozoa are a hub for diverse hydrogenotrophic functions.Environmental microbiology reports · 2024Article
- Buccal Swab Samples from Japanese Brown Cattle Fed with Limonite Reveal Altered Rumen Microbiome.Animals : an open access journal from MDPI · 2024Article
- Integrating microbial abundance time series with fermentation dynamics of the rumen microbiome via mathematical modelling.PloS one · 2024Article
- Fecal microbiota colonization dynamics in dairy heifers associated with early-life rumen microbiota modulation and gut health.Frontiers in microbiology · 2024Article
- Oral swabs as a proxy for direct ruminal microbiome sampling in Holstein dairy cows is correlated with sample color.Frontiers in microbiology · 2024Article
- Gut microbiome biogeography in reindeer supersedes millennia of ecological and evolutionary separation.FEMS microbiology ecology · 2023Article
- Ruminant Salivary Microbes: Passenger or Player in the Rumen?Microorganisms · 2023Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microbial community analysis was carried out on ruminal digesta obtained directly via rumen fistula and buccal fluid, regurgitated digesta (bolus) and faeces of dairy cattle to assess if non-invasive samples could be used as proxies for ruminal digesta. Samples were collected from five cows receiving grass silage based diets containing no additional lipid or four different lipid supplements in a 5 x 5 Latin square design. Extracted DNA was analysed by qPCR and by sequencing 16S and 18S rRNA genes or the fungal ITS1 amplicons. Faeces contained few protozoa, and bacterial, fungal and archaeal communities were substantially different to ruminal digesta. Buccal and bolus samples gave much more similar profiles to ruminal digesta, although fewer archaea were detected in buccal and bolus samples. Bolus samples overall were most similar to ruminal samples. The differences between both buccal and bolus samples and ruminal digesta were consistent across all treatments. It can be concluded that either proxy sample type could be used as a predictor of the rumen microbial community, thereby enabling more convenient large-scale animal sampling for phenotyping and possible use in future animal breeding programs aimed at selecting cattle with a lower environmental footprint.
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