ArticleJournal of clinical microbiology2016
Evaluation of Molecular Methods for Serotyping Shigella flexneri.
Article in Journal of clinical microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 44 citations in OpenAlex.
- DNAzyme-based colorimetric biosensor for rapid detection of Shigella flexneri.Scientific reports · 2025Article
- Article
- Microbiological Methods Used in the Enterics for Global HealthOpen forum infectious diseases · 2024Article
- Review
- ShigaPass: anMicrobial genomics · 2023Article
- Discrimination of Escherichia coli, Shigella flexneri, and Shigella sonnei using lipid profiling by MALDI-TOF mass spectrometry paired with machine learning.MicrobiologyOpen · 2022Article
- Virulence genes expression profiling of different Shigella flexneri serotypes in response to sub-inhibitory concentrations of azithromycin and ciprofloxacin.Gut pathogens · 2022Article
- CRISPR/Cas12a-Enhanced Loop-Mediated Isothermal Amplification for the Visual Detection ofFrontiers in bioengineering and biotechnology · 2022Article
- The evolutionary history ofMicrobial genomics · 2021Article
- Historical, current, and emerging tools for identification and serotyping of Shigella.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Review
- Evaluation of whole-genome sequencing-based subtyping methods for the surveillance ofMicrobial genomics · 2021Article
- Phylogenomic Investigation of Increasing Fluoroquinolone Resistance among Belgian Cases of Shigellosis between 2013 and 2018 Indicates Both Travel-Related Imports and Domestic Circulation.Microorganisms · 2021Article
- Evolutionary histories and antimicrobial resistance in Shigella flexneri and Shigella sonnei in Southeast Asia.Communications biology · 2021Article
- Evaluation of Molecular Serotyping Assays for Shigella flexneri Directly on Stool Samples.Journal of clinical microbiology · 2021Article
- Shigella sonnei: virulence and antibiotic resistance.Archives of microbiology · 2021Review
- Genomic Serotyping, Clinical Manifestations, and Antimicrobial Resistance of NontyphoidalJournal of clinical microbiology · 2020Article
- Shigella flexneri: an emerging pathogen.Folia microbiologica · 2020Review
- Reverse vaccinology approach for the identification and characterization of outer membrane proteins ofClinical and experimental vaccine research · 2020Article
- Imaging Inflammation and Infection in the Gastrointestinal Tract.International journal of molecular sciences · 2019Review
- Shifting national surveillance of Shigella infections toward geno-serotyping by the development of a tailored Luminex assay and NGS workflow.MicrobiologyOpen · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Shigella flexneri can be phenotypically serotyped using antisera raised to type-specific somatic antigens and group factor antigens and genotypically serotyped using PCR targeting O-antigen synthesis or modification genes. The aim of this study was to evaluate a real-time PCR for serotyping S. flexneri and to use whole-genome sequencing (WGS) to investigate the phenotypic and genotypic serotype identifications. Of the 244 cultures tested retrospectively, 226 (92.6%) had concordant results between phenotypic serotyping and PCR. Seventy of the 244 isolates (including 15 of the 18 isolates where a serotype-PCR mismatch was identified) were whole-genome sequenced, and the serotype was derived from the genome. Discrepant results between the phenotypic and genotypic tests were attributed to insertions/deletions or point mutations identified in O-antigen synthesis or modification genes, rendering them dysfunctional; inconclusive serotyping results due to nonspecific cross-reactions; or novel genotypes. Phylogenetic analysis of the WGS data indicated that the serotype, regardless of whether it was phenotypically or genotypically determined, was a weak predictor of phylogenetic relationships between strains of S. flexneri WGS data provided both genome-derived serotyping, thus supporting backward compatibility with historical data and facilitating data exchange in the community, and more robust and discriminatory typing at the single-nucleotide-polymorphism level.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.