ArticleAmerican journal of physiology. Cell physiology2016
Human vascular endothelial cells transport foreign exosomes from cow's milk by endocytosis.
Article in American journal of physiology. Cell physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 112 papers.
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Who cites it
112 citing papers in PubMed.
- Investigating Dietary microRNA Stability and Function Using a Transgenic Milk Model With Unique microRNA Sequences.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- Cross-Species Analysis of Milk Extracellular Vesicles Reveals a Conserved Innate Core and a Divergent, Human-Specific Adaptive Immune Fraction.International journal of molecular sciences · 2026Article
- Conserved Core and Species-Specific Signatures in the Milk Exosomal microRNA Targetome: A Preliminary Comparative In Silico Analysis of Human, Cow, Goat and Donkey Milk.International journal of molecular sciences · 2026Article
- Article
- Look Who Is Talking: Extracellular Vesicles as Mediators of Intercellular and Interorgan Communication.Comprehensive Physiology · 2026Review
- A novel spray-dried milk extracellular vesicles formulation with long-term immunomodulatory activity and functional stability.Scientific reports · 2026Article
- Exploiting the role of milk extracellular vesicles: a comprehensive analysis on isolation methods, characterization, surface modifications, and their therapeutic applications.Journal of nanobiotechnology · 2026Review
- Molecular composition of meats: a novel look at microRNAs as functional nutrients.Animal frontiers : the review magazine of animal agriculture · 2026Article
- Characterization and Comparison of Milk Fat Globule Membrane Proteins, Whey Protein Concentrate, and Micellar Casein Concentrate.Food science & nutrition · 2026Article
- First insights into the miRNA landscape of Tursiops truncatus milk reveal shared dominant microRNA families with terrestrial mammals.PloS one · 2026Article
- Comparison of Different Encapsulation Techniques in Exosomes Obtained from Goat and Bovine Milk.ACS omega · 2025Article
- The Promise of Exosomes in Cardiac Repair: When 'Best Things Come in Small Packages'.Journal of cellular and molecular medicine · 2025Review
- Comparative Proteomic Analysis of Extracellular Vesicles from Donkey Colostrum and Mature Milk.Metabolites · 2025Article
- Article
- Milk-Derived Extracellular Vesicles and microRNAs: Potential Modulators of Intestinal Homeostasis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Review
- Diabetes-preventive molecular mechanisms of breast versus formula feeding: new insights into the impact of milk on stem cell Wnt signaling.Frontiers in nutrition · 2025Review
- Untargeted lipidomics reveals unique lipid signatures of extracellular vesicles from porcine colostrum and milk.PloS one · 2025Article
- Inorganic Nanoparticles-based Drug Delivery Systems for Neurodegenerative Diseases Therapy.Current pharmaceutical design · 2025Review
- Unlocking the Potential of Camel Milk-Derived Exosomes as Novel Delivery Systems: Enhanced Bioavailability of ARV-825 PROTAC for Cancer Therapy.Pharmaceutics · 2024Article
- Comparison of colostrum and milk extracellular vesicles small RNA cargo in water buffalo.Scientific reports · 2024Article
52 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors.
Funding
Abstract
Encapsulation of microRNAs in exosomes confers protection against degradation and a vehicle for shuttling of microRNAs between cells and tissues, and cellular uptake by endocytosis. Exosomes can be found in foods including milk. Humans absorb cow's milk exosomes and deliver the microRNA cargo to peripheral tissues, consistent with gene regulation by dietary nucleic acids across species boundaries. Here, we tested the hypothesis that human vascular endothelial cells transport milk exosomes by endocytosis, constituting a step crucial for the delivery of dietary exosomes and their cargo to peripheral tissues. We tested this hypothesis by using human umbilical vein endothelial cells and fluorophore-labeled exosomes isolated from cow's milk. Exosome uptake followed Michaelis-Menten kinetics (Vmax = 0.057 ± 0.004 ng exosome protein × 40,000 cells/h; Km = 17.97 ± 3.84 μg exosomal protein/200 μl media) and decreased by 80% when the incubation temperature was lowered from 37°C to 4°C. When exosome surface proteins were removed by treatment with proteinase K, or transport was measured in the presence of the carbohydrate competitor d-galactose or measured in the presence of excess unlabeled exosomes, transport rates decreased by 45% to 80% compared with controls. Treatment with an inhibitor of endocytosis, cytochalasin D, caused a 50% decrease in transport. When fluorophore-labeled exosomes were administered retro-orbitally, exosomes accumulated in liver, spleen, and lungs in mice. We conclude that human vascular endothelial cells transport bovine exosomes by endocytosis and propose that this is an important step in the delivery of dietary exosomes and their cargo to peripheral tissues.
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