Evidence map›Paper›PMID 26984640›Full record

ArticleJournal of nanobiotechnology2016

Bioluminescent magnetic nanoparticles as potential imaging agents for mammalian spermatozoa.

Erick S Vasquez, Jean M Feugang, Scott T Willard, Peter L Ryan, Keisha B Walters

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of nanobiotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Article
  5. Article
  6. Review
  7. Review
  8. Indirect magnetic force microscopy.Nanoscale advances · 2019
    Article
  9. Article
  10. Article
  11. Non-Invasive Approaches to Epigenetic-Based Sperm Selection.Medical science monitor : international medical journal of experimental and clinical research · 2017
    Review
  12. Current and future prospects for nanotechnology in animal production.Journal of animal science and biotechnology · 2017
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Erick S VasquezDepartment of Chemical and Materials Engineering, University of Dayton, Dayton, OH, 45469, USA.
Jean M FeugangFacility for Cellular and Organismal Imaging, Mississippi State University, Mississippi State, MS, 39762, USA. jn181@ads.msstate.edu.ORCID http://orcid.org/0000-0002-0875-2659
Scott T WillardFacility for Cellular and Organismal Imaging, Mississippi State University, Mississippi State, MS, 39762, USA.
Peter L RyanFacility for Cellular and Organismal Imaging, Mississippi State University, Mississippi State, MS, 39762, USA.
Keisha B WaltersDave C. Swalm School of Chemical Engineering, Mississippi State University, Mississippi State, MS, 39762, USA. kwalters@che.msstate.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundNanoparticles have emerged as key materials for developing applications in nanomedicine, nanobiotechnology, bioimaging and theranostics. Existing bioimaging technologies include bioluminescent resonance energy transfer-conjugated quantum dots (BRET-QDs). Despite the current use of BRET-QDs for bioimaging, there are strong concerns about QD nanocomposites containing cadmium which exhibits potential cellular toxicity.

resultsIn this study, bioluminescent composites comprised of magnetic nanoparticles and firefly luciferase (Photinus pyralis) are examined as potential light-emitting agents for imaging, detection, and tracking mammalian spermatozoa. Characterization was carried out using infrared spectroscopy, TEM and cryo-TEM imaging, and ζ-potential measurements to demonstrate the successful preparation of these nanocomposites. Binding interactions between the synthesized nanoparticles and spermatozoon were characterized using confocal and atomic/magnetic force microscopy. Bioluminescence imaging and UV-visible-NIR microscopy results showed light emission from sperm samples incubated with the firefly luciferase-modified nanoparticles. Therefore, these newly synthesized luciferase-modified magnetic nanoparticles show promise as substitutes for QD labeling, and can potentially also be used for in vivo manipulation and tracking, as well as MRI techniques.

conclusionsThese preliminary data indicate that luciferase-magnetic nanoparticle composites can potentially be used for spermatozoa detection and imaging. Their magnetic properties add additional functionality to allow for manipulation, sorting, or tracking of cells using magnetic techniques.

Indexed as

AnimalsDiagnostic ImagingLuciferasesLuminescent MeasurementsMagneticsMagnetite NanoparticlesMaleNanocompositesQuantum DotsSpermatozoaSwineLuciferasesMagnetite NanoparticlesBioluminescence ImagingCore–shell nanoparticlesLuciferaseMagnetic nanoparticlesNanocompositesReproductionSpermatozoa

Identifiers

PMID26984640
PMCPMC4794913

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.