ArticleGenetics2016
Persistence of RNAi-Mediated Knockdown in Drosophila Complicates Mosaic Analysis Yet Enables Highly Sensitive Lineage Tracing.
Article in Genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
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- An interplay between cellular growth and atypical fusion defines morphogenesis of a modular glial niche in Drosophila.Nature communications · 2022Article
- Intermediate progenitor cells provide a transition between hematopoietic progenitors and their differentiated descendants.Development (Cambridge, England) · 2021Article
- Developmental expression patterns of toolkit genes in male accessory gland of Drosophila parallels those of mammalian prostate.Biology open · 2021Article
- me31B regulates stem cell homeostasis by preventing excess dedifferentiation in the Drosophila male germline.Journal of cell science · 2021Article
- Piwi reduction in the aged niche eliminates germline stem cells via Toll-GSK3 signaling.Nature communications · 2020Article
- Model systems for regeneration:Development (Cambridge, England) · 2020Review
- Monitoring of switches in heterochromatin-induced silencing shows incomplete establishment and developmental instabilities.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- RNA Interference (RNAi) Screening inGenetics · 2018Article
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- The Drosophila Duox maturation factor is a key component of a positive feedback loop that sustains regeneration signaling.PLoS genetics · 2017Article
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- The Narrow Abdomen Ion Channel Complex Is Highly Stable and Persists from Development into Adult Stages to Promote Behavioral Rhythmicity.Frontiers in cellular neuroscience · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
RNA interference (RNAi) has emerged as a powerful way of reducing gene function in Drosophila melanogaster tissues. By expressing synthetic short hairpin RNAs (shRNAs) using the Gal4/UAS system, knockdown is efficiently achieved in specific tissues or in clones of marked cells. Here we show that knockdown by shRNAs is so potent and persistent that even transient exposure of cells to shRNAs can reduce gene function in their descendants. When using the FLP-out Gal4 method, in some instances we observed unmarked "shadow RNAi" clones adjacent to Gal4-expressing clones, which may have resulted from brief Gal4 expression following recombination but prior to cell division. Similarly, Gal4 driver lines with dynamic expression patterns can generate shadow RNAi cells after their activity has ceased in those cells. Importantly, these effects can lead to erroneous conclusions regarding the cell autonomy of knockdown phenotypes. We have investigated the basis of this phenomenon and suggested experimental designs for eliminating ambiguities in interpretation. We have also exploited the persistence of shRNA-mediated knockdown to design a sensitive lineage-tracing method, i-TRACE, which is capable of detecting even low levels of past reporter expression. Using i-TRACE, we demonstrate transient infidelities in the expression of some cell-identity markers near compartment boundaries in the wing imaginal disc.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.