ArticleAnnals of biomedical engineering2016
Understanding the Role of ECM Protein Composition and Geometric Micropatterning for Engineering Human Skeletal Muscle.
Article in Annals of biomedical engineering, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
41 citing papers in PubMed, 96 citations in OpenAlex.
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- Limb-on-a-Chip: An All-Hydrogel Platform for Scalable and Reproducible Engineering of Functional Neuromuscular Tissues.Cell biomaterials · 2026Article
- 2D Skeletal Muscle Thin Film Actuators Enhance Efficiency of Biohybrid Robots.bioRxiv : the preprint server for biology · 2026Article
- Physiological and functional characterization for high-throughput optogenetic skeletal muscle exercise assays.Bioengineering & translational medicine · 2026Article
- Chick embryo extract-driven culture system enhances chicken muscle satellite cell differentiation and 3D myotube formation.NPJ science of food · 2025Article
- Enhancing Skeletal Muscle Fiber Type Transition Through Substrate Coating Alteration in Myoblast Cell Culture.International journal of molecular sciences · 2025Article
- Leveraging microtopography to pattern multi-oriented muscle actuators.Biomaterials science · 2025Article
- Enhancing Viability in Static and Perfused 3D Tissue Constructs Using Sacrificial Gelatin Microparticles.ACS biomaterials science & engineering · 2025Article
- Actuating Extracellular Matrices Decouple the Mechanical and Biochemical Effects of Muscle Contraction on Motor Neurons.Advanced healthcare materials · 2025Article
- Growing meat on autoclaved vegetables with biomimetic stiffness and micro-patterns.Nature communications · 2025Article
- Skeletal muscle cells opto-stimulation by intramembrane molecular transducers.Communications biology · 2023Article
- Combinatorial extracellular matrix cues with mechanical strain induce differential effects on myogenesisBiomaterials science · 2023Article
- Instant formation of horizontally ordered nanofibrous hydrogel films and direct investigation of peculiar neuronal cell behaviors atop.Biomaterials research · 2023Article
- Serum-free alginate-C2C12 cells microcapsule as a model of alternative animal protein source.Frontiers in nutrition · 2023Article
- 3D Bioprinted Patient-Specific Extracellular Matrix Scaffolds for Soft Tissue Defects.Advanced healthcare materials · 2022Article
- Review
- Matrix produced by diseased cardiac fibroblasts affects early myotube formation and function.Acta biomaterialia · 2022Article
- Myocyte Culture with Decellularized Skeletal Muscle Sheet with Observable Interaction with the Extracellular Matrix.Bioengineering (Basel, Switzerland) · 2022Article
- The Emerging Role of Decellularized Plant-Based Scaffolds as a New Biomaterial.International journal of molecular sciences · 2021Review
- Replace and repair: Biomimetic bioprinting for effective muscle engineering.APL bioengineering · 2021Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
Abstract
Skeletal muscle lost through trauma or disease has proven difficult to regenerate due to the challenge of differentiating human myoblasts into aligned, contractile tissue. To address this, we investigated microenvironmental cues that drive myoblast differentiation into aligned myotubes for potential applications in skeletal muscle repair, organ-on-chip disease models and actuators for soft robotics. We used a 2D in vitro system to systematically evaluate the role of extracellular matrix (ECM) protein composition and geometric patterning for controlling the formation of highly aligned myotubes. Specifically, we analyzed myotubes differentiated from murine C2C12 cells and human skeletal muscle derived cells (SkMDCs) on micropatterned lines of laminin compared to fibronectin, collagen type I, and collagen type IV. Results showed that laminin supported significantly greater myotube formation from both cells types, resulting in greater than twofold increase in myotube area on these surfaces compared to the other ECM proteins. Species specific differences revealed that human SkMDCs uniaxially aligned over a wide range of micropatterned line dimensions, while C2C12s required specific line widths and spacings to do the same. Future work will incorporate these results to engineer aligned human skeletal muscle tissue in 2D for in vitro applications in disease modeling, drug discovery and toxicity screening.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.