Evidence map›Paper›PMID 26983800›Full record

ArticleScientific reports2016

A new hand-held microfluidic cytometer for evaluating irradiation damage by analysis of the damaged cells distribution.

Junsheng Wang, Zhiqiang Fan, Yile Zhao, Younan Song, Hui Chu, Wendong Song, Yongxin Song, Xinxiang Pan, Yeqing Sun, Dongqing Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Junsheng WangCollege of Information and Science Technology, Dalian Maritime University, Dalian, 116026, China.
Zhiqiang FanCollege of Information and Science Technology, Dalian Maritime University, Dalian, 116026, China.
Yile ZhaoCollege of Information and Science Technology, Dalian Maritime University, Dalian, 116026, China.
Younan SongCollege of Information and Science Technology, Dalian Maritime University, Dalian, 116026, China.
Hui ChuCollege of Information and Science Technology, Dalian Maritime University, Dalian, 116026, China.
Wendong SongCollege of Information and Science Technology, Dalian Maritime University, Dalian, 116026, China.
Yongxin SongCollege of Marine Engineering, Dalian Maritime University, Dalian, 116026, China.
Xinxiang PanCollege of Marine Engineering, Dalian Maritime University, Dalian, 116026, China.
Yeqing SunCollege of Environmental Science and Engineering, Dalian Maritime University, Dalian, 116026, China.
Dongqing LiCollege of Marine Engineering, Dalian Maritime University, Dalian, 116026, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Space radiation brings uneven damages to cells. The detection of the distribution of cell damage plays a very important role in radiation medicine and the related research. In this paper, a new hand-held microfluidic flow cytometer was developed to evaluate the degree of radiation damage of cells. The device we propose overcomes the shortcomings (e.g., large volume and high cost) of commercial flow cytometers and can evaluate the radiation damage of cells accurately and quickly with potential for onsite applications. The distribution of radiation-damaged cells is analyzed by a simultaneous detection of immunofluorescence intensity of γ-H2AX and resistance pulse sensor (RPS) signal. The γ-H2AX fluorescence intensity provides information of the degree of radiation damage in cells. The ratio of the number of cells with γ-H2AX fluorescence signals to the total numbers of cells detected by RPS indicates the percentage of the cells that are damaged by radiation. The comparison experiment between the developed hand-held microfluidic flow cytometer and a commercial confocal microscope indicates a consistent and comparable detection performance.

Indexed as

Ultraviolet RaysFlow CytometryFluorescent DyesHistonesHumansLymphocytesMicrofluidic Analytical TechniquesMicroscopy, FluorescencePhosphorylationFluorescent DyesH2AX protein, humanHistones

Identifiers

PMID26983800
PMCPMC4794725

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.