ArticleNanoscale research letters2016
Bioprobes Based on Aptamer and Silica Fluorescent Nanoparticles for Bacteria Salmonella typhimurium Detection.
Article in Nanoscale research letters, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Recent Progress in Nanomaterial-Based Fluorescence Assays for the Detection of Food-Borne Pathogens.Sensors (Basel, Switzerland) · 2024Review
- Trends in Development of Aptamer-Based Biosensor Technology for Detection of Bacteria.Advances in biochemical engineering/biotechnology · 2024Review
- A Colorimetric/Fluorescent Dual-Mode Aptasensor forFoods (Basel, Switzerland) · 2023Article
- Optimized antibody immobilization on natural silica-based nanostructures for the selective detection ofRSC advances · 2022Article
- A Specific and Sensitive Aptamer-Based Digital PCR Chip forBiosensors · 2022Article
- Nucleic Acid-Based Nanobiosensor (NAB) Used forNanomaterials (Basel, Switzerland) · 2022Review
- Development and Evaluation of Fluorescence Immunochromatography for Rapid and Sensitive Detection of ThermophilicFood safety (Tokyo, Japan) · 2021Article
- Advances in Laser Ablation Synthesized Silicon-Based Nanomaterials for the Prevention of Bacterial Infection.Nanomaterials (Basel, Switzerland) · 2020Review
- Nanomaterials for Biosensing Lipopolysaccharide.Biosensors · 2019Review
- Article
- Oligonucleotide aptamers: promising and powerful diagnostic and therapeutic tools for infectious diseases.The Journal of infection · 2018Review
- Aptamer Combined with Fluorescent Silica Nanoparticles for Detection of Hepatoma Cells.Nanoscale research letters · 2017Article
- A New Theranostic System Based on Endoglin Aptamer Conjugated Fluorescent Silica Nanoparticles.Theranostics · 2017Article
- Potentiometric Aptasensing of Vibrio alginolyticus Based on DNA Nanostructure-Modified Magnetic Beads.Sensors (Basel, Switzerland) · 2016Article
- Advanced Nanobiomaterials: Vaccines, Diagnosis and Treatment of Infectious Diseases.Molecules (Basel, Switzerland) · 2016Review
Corrections and comments
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Authors and funding
2 authors.
Funding
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Abstract
In this study, we have developed an efficient method based on single-stranded DNA (ssDNA) aptamers along with silica fluorescence nanoparticles for bacteria Salmonella typhimurium detection. Carboxyl-modified Tris(2,2'-bipyridyl)dichlororuthenium(II) hexahydrate (RuBPY)-doped silica nanoparticles (COOH-FSiNPs) were prepared using reverse microemulsion method, and the streptavidin was conjugated to the surface of the prepared COOH-FSiNPs. The bacteria S. typhimurium was incubated with a specific ssDNA biotin-labeled aptamer, and then the aptamer-bacteria conjugates were treated with the synthetic streptavidin-conjugated silica fluorescence nanoprobes (SA-FSiNPs). The results under fluorescence microscopy show that SA-FSiNPs can be applied effectively for the labeling of bacteria S. typhimurium with great photostable property. To further verify the specificity of SA-FSiNPs out of multiple bacterial conditions, variant concentrations of bacteria mixtures composed of bacteria S. typhimurium, Escherichia coli, and Bacillus subtilis were treated with SA-FSiNPs.In addition, the feasibility of SA-FSiNPs for bacteria S. typhimurium detection in chicken samples was assessed. All the results display that the established aptamer-based nanoprobes exhibit the superiority for bacteria S. typhimurium detection, which is referentially significant for wider application prospects in pathogen detection.
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