ArticlePloS one2016
Collagen-Hydroxyapatite Scaffolds Induce Human Adipose Derived Stem Cells Osteogenic Differentiation In Vitro.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
68 citing papers in PubMed, 125 citations in OpenAlex.
- Nanoparticle-Enabled Modulation of the Bone Immune Microenvironment for Enhanced Regeneration.Bioengineering (Basel, Switzerland) · 2026Review
- Fucoidan in cancer therapy: from biomedical application to medicinal chemistry approach.Journal of materials science. Materials in medicine · 2025Review
- Core-shell silica and fluorogenic hyaluronan nanomaterials in magnesium hydroxyapatite scaffolds for bone regeneration.Scientific reports · 2025Article
- Intraoperative Bioprinting for Craniomaxillofacial Bone Reconstruction in Rats and Sheep.Small science · 2025Article
- Magnesium Ion-Mediated Regulation of Osteogenesis and Osteoclastogenesis in 2D Culture and 3D Collagen/Nano-Hydroxyapatite Scaffolds for Enhanced Bone Repair.Journal of functional biomaterials · 2025Article
- Hydroxyapatite Scaffold and Bioactive Factor Combination as a Tool to Improve Osteogenesis, In Vitro and In Vivo Experiments Using Phage Display Technology.International journal of molecular sciences · 2025Article
- Injectable Dendritic Hydrogels Curable by High-Energy Visible Light for Cell Delivery in Bone Regeneration.Chemistry of materials : a publication of the American Chemical Society · 2025Article
- Magnetoactive Nanotopography on Hydrogels for Stimulated Cell Adhesion and Differentiation.Small science · 2025Article
- ECM Modifications Driven by Age and Metabolic Stress Directly Promote Vascular Smooth Muscle Cell Osteogenic Processes.Arteriosclerosis, thrombosis, and vascular biology · 2025Article
- Role of Adipose-Derived Mesenchymal Stem Cells in Bone Regeneration.International journal of molecular sciences · 2024Review
- Bone Tissue Engineering and Nanotechnology: A Promising Combination for Bone Regeneration.Biology · 2024Review
- Sintered fluorapatite scaffolds as an autograft-like engineered bone graft.Journal of biomedical materials research. Part B, Applied biomaterials · 2024Article
- Collagen-hydroxyapatite based scaffolds for bone trauma and regeneration: recent trends and future perspectives.Nanomedicine (London, England) · 2024Review
- Bioprinting of gelatin-based materials for orthopedic application.Frontiers in bioengineering and biotechnology · 2024Review
- Biomimetic Inspired Hydrogels for Regenerative Vertebral Body Stenting.Current osteoporosis reports · 2023Review
- Enhancing bone tissue engineering with 3D-Printed polycaprolactone scaffolds integrated with tragacanth gum/bioactive glass.Materials today. Bio · 2023Article
- 3D and 4D printing hydroxyapatite-based scaffolds for bone tissue engineering and regeneration.Heliyon · 2023Review
- In situ self-assembled organoid for osteochondral tissue regeneration with dual functional units.Bioactive materials · 2023Article
- Review
- A 3D-Printed Biomaterial Scaffold Reinforced with Inorganic Fillers for Bone Tissue Engineering: In Vitro Assessment and In Vivo Animal Studies.International journal of molecular sciences · 2023Article
8 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mesenchymal stem cells (MSCs) play a crucial role in regulating normal skeletal homeostasis and, in case of injury, in bone healing and reestablishment of skeletal integrity. Recent scientific literature is focused on the development of bone regeneration models where MSCs are combined with biomimetic three-dimensional scaffolds able to direct MSC osteogenesis. In this work the osteogenic potential of human MSCs isolated from adipose tissue (hADSCs) has been evaluated in vitro in combination with collagen/Mg doped hydroxyapatite scaffolds. Results demonstrate the high osteogenic potential of hADSCs when cultured in specific differentiation induction medium, as revealed by the Alizarin Red S staining and gene expression profile analysis. In combination with collagen/hydroxyapatite scaffold, hADSCs differentiate into mature osteoblasts even in the absence of specific inducing factors; nevertheless, the supplement of the factors markedly accelerates the osteogenic process, as confirmed by the expression of specific markers of pre-osteoblast and mature osteoblast stages, such as osterix, osteopontin (also known as bone sialoprotein I), osteocalcin and specific markers of extracellular matrix maturation and mineralization stages, such as ALPL and osteonectin. Hence, the present work demonstrates that the scaffold per se is able to induce hADSCs differentiation, while the addition of osteo-inductive factors produces a significant acceleration of the osteogenic process. This observation makes the use of our model potentially interesting in the field of regenerative medicine for the treatment of bone defects.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.