ArticlePloS one2016
Detection of Mycobacterium ulcerans DNA in the Environment, Ivory Coast.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 44 citations in OpenAlex.
- Risk factors for Buruli ulcer disease in Ghana: A matched case-control study in four selected endemic districts of Eastern and Oti Regions.PLoS neglected tropical diseases · 2025Article
- Suspected Buruli ulcer cases in Tonkolili District, Sierra Leone- a prospective cohort study.Infection · 2025Article
- Systematic review: Global host range, case fatality and detection rates ofJournal of clinical tuberculosis and other mycobacterial diseases · 2024Review
- Floristic Diversity as an Indicator in Low and High Endemic Buruli Ulcer Areas in Côte d'Ivoire.International journal of environmental research · 2023Article
- A combined field study of Buruli ulcer disease in southeast Benin proposing preventive strategies based on epidemiological, geographic, behavioural and environmental analyses.PLOS global public health · 2022Article
- Linking theOne health (Amsterdam, Netherlands) · 2021Review
- Community-based geographical distribution of Mycobacterium ulcerans VNTR-genotypes from the environment and humans in the Nyong valley, Cameroon.Tropical medicine and health · 2021Article
- Introduction of Mycobacterium ulcerans disease in the Bankim Health District of Cameroon follows damming of the Mapé River.PLoS neglected tropical diseases · 2020Article
- Tracing Mycobacterium ulcerans along an alimentary chain in Côte d'Ivoire: A one health perspective.PLoS neglected tropical diseases · 2020Article
- Screening anti-infectious molecules against Mycobacterium ulcerans: A step towards decontaminating environmental specimens.PloS one · 2020Article
- DisseminatedThe American journal of tropical medicine and hygiene · 2019Article
- The changing epidemiology worldwide ofEpidemiology and infection · 2018Article
- Domestic animals infected with Mycobacterium ulcerans-Implications for transmission to humans.PLoS neglected tropical diseases · 2018Article
- The Role of aDNA in Understanding the Coevolutionary Patterns of Human Sexually Transmitted Infections.Genes · 2018Review
- Potential Animal Reservoir ofTropical medicine and infectious disease · 2018Review
- Molecular detection of Mycobacterium ulcerans in the environment and its relationship with Buruli ulcer occurrence in Zio and Yoto districts of maritime region in Togo.PLoS neglected tropical diseases · 2018Article
- A protocol for culturing environmental strains of the Buruli ulcer agent, Mycobacterium ulcerans.Scientific reports · 2018Article
- Comparative Genomics Shows That Mycobacterium ulcerans Migration and Expansion Preceded the Rise of Buruli Ulcer in Southeastern Australia.Applied and environmental microbiology · 2018Article
- Risk factors for Mycobacterium ulcerans infection (Buruli Ulcer) in Togo ─ a case-control study in Zio and Yoto districts of the maritime region.BMC infectious diseases · 2018Article
- Buruli Ulcer: a Review of the Current Knowledge.Current tropical medicine reports · 2018Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIvory Coast is a West African country with the highest reported cases of Buruli ulcer, a disabling subcutaneous infection due to Mycobacterium ulcerans. However, the prevalence of environmental M. ulcerans is poorly known in this country.
methodsWe collected 496 environmental specimens consisting of soil (n = 100), stagnant water (n = 200), plants (n = 100) and animal feces (n = 96) in Ivory Coast over five months in the dry and wet seasons in regions which are free of Buruli ulcer (control group A; 250 specimens) and in regions where the Buruli ulcer is endemic (group B; 246 specimens). After appropriate total DNA extraction incorporating an internal control, the M. ulcerans IS2404 and KR-B gene were amplified by real-time PCR in samples. In parallel, a calibration curve was done for M. ulcerans Agy99 IS2404 and KR-B gene.
resultsOf 460 samples free of PCR inhibition, a positive real-time PCR detection of insertion sequence IS2404 and KR-B gene was observed in 1/230 specimens in control group A versus 9/230 specimens in group B (P = 0.02; Fisher exact test). Positive specimens comprised seven stagnant water specimens, two feces specimens confirmed to be of Thryonomys swinderianus (agouti) origin by real-time PCR of the cytb gene; and one soil specimen. Extrapolation from the calibration curves indicated low inoculums ranging from 1 to 102 mycobacteria/mL.
conclusionThis study confirms the presence of M. ulcerans in the watery environment surrounding patients with Buruli ulcer in Ivory Coast. It suggests that the agouti, which is in close contacts with populations, could play a role in the environmental cycle of M. ulcerans, as previously suggested for the closely related possums in Australia.
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