ArticlePloS one2016
An Approach to Function Annotation for Proteins of Unknown Function (PUFs) in the Transcriptome of Indian Mulberry.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Comprehensive Analysis of Universal Stress Protein Family Genes and Their Expression inInternational journal of molecular sciences · 2023Article
- A BTB-TAZ protein is required for gene activation by Cauliflower mosaic virus 35S multimerized enhancers.Plant physiology · 2022Article
- In-Silico Functional Annotation ofFrontiers in genetics · 2022Article
- Comparative Proteomic Analysis of Tolerant and Sensitive Varieties Reveals That Phenylpropanoid Biosynthesis Contributes to Salt Tolerance in Mulberry.International journal of molecular sciences · 2021Article
- Defense Response in Chickpea Pod Wall due to Simulated Herbivory Unfolds Differential Proteome Profile.The protein journal · 2020Article
- An endophyte from salt-adapted Pokkali rice confers salt-tolerance to a salt-sensitive rice variety and targets a unique pattern of genes in its new host.Scientific reports · 2020Article
- Overexpression of a Novel Arabidopsis GeneFrontiers in plant science · 2020Article
- OPENER Is a Nuclear Envelope and Mitochondria Localized Protein Required for Cell Cycle Progression in Arabidopsis.The Plant cell · 2019Article
- Transcriptome analysis of finger millet (Journal of genetics · 2019Article
- Physiological and Proteomic Responses of Mulberry Trees (International journal of molecular sciences · 2019Article
- Analysis of Protein Complexes in the Unicellular Cyanobacterium Cyanothece ATCC 51142.Journal of proteome research · 2018Article
- Identification of water use efficiency related genes in 'Garnem' almond-peach rootstock using time-course transcriptome analysis.PloS one · 2018Article
- Mulberry (Morus spp.) has the features to treat as a potential perennial model system.Plant signaling & behavior · 2018Review
- Improvement of abiotic stress adaptive traits in mulberry (Morus spp.): an update on biotechnological interventions.3 Biotech · 2017Review
- Article
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Authors and funding
7 authors.
Funding
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Abstract
The modern sequencing technologies are generating large volumes of information at the transcriptome and genome level. Translation of this information into a biological meaning is far behind the race due to which a significant portion of proteins discovered remain as proteins of unknown function (PUFs). Attempts to uncover the functional significance of PUFs are limited due to lack of easy and high throughput functional annotation tools. Here, we report an approach to assign putative functions to PUFs, identified in the transcriptome of mulberry, a perennial tree commonly cultivated as host of silkworm. We utilized the mulberry PUFs generated from leaf tissues exposed to drought stress at whole plant level. A sequence and structure based computational analysis predicted the probable function of the PUFs. For rapid and easy annotation of PUFs, we developed an automated pipeline by integrating diverse bioinformatics tools, designated as PUFs Annotation Server (PUFAS), which also provides a web service API (Application Programming Interface) for a large-scale analysis up to a genome. The expression analysis of three selected PUFs annotated by the pipeline revealed abiotic stress responsiveness of the genes, and hence their potential role in stress acclimation pathways. The automated pipeline developed here could be extended to assign functions to PUFs from any organism in general. PUFAS web server is available at http://caps.ncbs.res.in/pufas/ and the web service is accessible at http://capservices.ncbs.res.in/help/pufas.
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