Evidence map›Paper›PMID 26981572›Full record

ArticleEBioMedicine2016

Mapping Human Pluripotent-to-Cardiomyocyte Differentiation: Methylomes, Transcriptomes, and Exon DNA Methylation "Memories".

Joshua D Tompkins, Marc Jung, Chang-Yi Chen, Ziguang Lin, Jingjing Ye, Swetha Godatha, Elizabeth Lizhar, Xiwei Wu, David Hsu, Larry A Couture and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in EBioMedicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed.

  1. Trial
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  15. Article
  16. Pten Regulates Cardiomyocyte Differentiation by Modulating Non-CG Methylation via Dnmt3.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021
    Article
  17. Article
  18. Minimal contribution of IPActa pharmacologica Sinica · 2020
    Article
  19. Polysome-associated lncRNAs during cardiomyogenesis of hESCs.Molecular and cellular biochemistry · 2020
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Joshua D TompkinsDepartment of Diabetes Complications and Metabolism, Duarte, CA 91010, USA; Beckman Research Institute at City of Hope National Medical Center, Duarte, CA 91010, USA.
Marc JungDepartment of Diabetes Complications and Metabolism, Duarte, CA 91010, USA; Beckman Research Institute at City of Hope National Medical Center, Duarte, CA 91010, USA.
Chang-Yi ChenCenter for Biomedicine and Genetics, Duarte, CA 91010, USA; Sylvia R. and Isador A. Deutch Center for Applied Technology Development, Duarte, CA 91010, USA; Beckman Research Institute at City of Hope National Medical Center, Duarte, CA 91010, USA.
Ziguang LinCenter for Biomedicine and Genetics, Duarte, CA 91010, USA; Sylvia R. and Isador A. Deutch Center for Applied Technology Development, Duarte, CA 91010, USA; Beckman Research Institute at City of Hope National Medical Center, Duarte, CA 91010, USA.
Jingjing YeCenter for Biomedicine and Genetics, Duarte, CA 91010, USA; Sylvia R. and Isador A. Deutch Center for Applied Technology Development, Duarte, CA 91010, USA; Beckman Research Institute at City of Hope National Medical Center, Duarte, CA 91010, USA.
Swetha GodathaDepartment of Diabetes Complications and Metabolism, Duarte, CA 91010, USA; Beckman Research Institute at City of Hope National Medical Center, Duarte, CA 91010, USA.
Elizabeth LizharDepartment of Diabetes Complications and Metabolism, Duarte, CA 91010, USA; Beckman Research Institute at City of Hope National Medical Center, Duarte, CA 91010, USA.
Xiwei WuBiomedical Informatics Core, Duarte, CA 91010, USA; Beckman Research Institute at City of Hope National Medical Center, Duarte, CA 91010, USA.
David HsuCenter for Biomedicine and Genetics, Duarte, CA 91010, USA; Sylvia R. and Isador A. Deutch Center for Applied Technology Development, Duarte, CA 91010, USA; Beckman Research Institute at City of Hope National Medical Center, Duarte, CA 91010, USA.
Larry A CoutureCenter for Biomedicine and Genetics, Duarte, CA 91010, USA; Sylvia R. and Isador A. Deutch Center for Applied Technology Development, Duarte, CA 91010, USA; Beckman Research Institute at City of Hope National Medical Center, Duarte, CA 91010, USA.
Arthur D RiggsDepartment of Diabetes Complications and Metabolism, Duarte, CA 91010, USA; Beckman Research Institute at City of Hope National Medical Center, Duarte, CA 91010, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The directed differentiation of human cardiomyocytes (CMs) from pluripotent cells provides an invaluable model for understanding mechanisms of cell fate determination and offers considerable promise in cardiac regenerative medicine. Here, we utilize a human embryonic stem cell suspension bank, produced according to a good manufacturing practice, to generate CMs using a fully defined and small molecule-based differentiation strategy. Primitive and cardiac mesoderm purification was used to remove non-committing and multi-lineage populations and this significantly aided the identification of key transcription factors, lncRNAs, and essential signaling pathways that define cardiomyogenesis. Global methylation profiles reflect CM development and we report on CM exon DNA methylation "memories" persisting beyond transcription repression and marking the expression history of numerous developmentally regulated genes, especially transcription factors.

Indexed as

DNA MethylationEpigenesis, GeneticExonsTranscriptomeCell DifferentiationCell LineageCells, CulturedEmbryonic Stem CellsHumansMyocytes, CardiacPluripotent Stem CellsCardiomyocytesCardiomyogenesisDifferentiationDNA methylationEpigeneticGood manufacturing practice, GMP, epigenetic memory, WNT, hedgehog, transforming growth factor, ROR2, PDGFRα, demethylation, TET, TDG, HOX, TBOXHuman embryonic stem cellslncRNALong non-coding RNAMesodermMethylomePluripotentTranscriptome

Identifiers

PMID26981572
PMCPMC4776252

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.