ArticleBMC genomics2016
Transcriptional profiling of Klebsiella pneumoniae defines signatures for planktonic, sessile and biofilm-dispersed cells.
Article in BMC genomics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Genome-scale metabolic modeling reveals increased reliance on valine catabolism in clinical isolates of Klebsiella pneumoniae.NPJ systems biology and applications · 2022Pooled it
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- Phenotypic and Transcriptomic Characterization of Host-Associated Responses in a Carbapenem-ResistantPathogens (Basel, Switzerland) · 2026Article
- Intestinal mucus acts as a nutrient source and signal forMicrobiome research reports · 2026Article
- Extracellular adherence proteins reduce matrix porosity and enhanceInfection and immunity · 2025Article
- Human PMNs exhibit greater engulfment, NETosis, and enhanced migration when incubated with nontypeableFrontiers in microbiology · 2025Article
- Matrix porosity is associated withbioRxiv : the preprint server for biology · 2024Article
- Deep Intraclonal Analysis for the Development of Vaccines against Drug-ResistantInternational journal of molecular sciences · 2024Article
- Genetically engineered phages and engineered phage-derived enzymes to destroy biofilms of antibiotics resistance bacteria.Heliyon · 2024Review
- Sniffing the wine differences: The role ofHeliyon · 2024Article
- Application of biofilm dispersion-based nanoparticles in cutting off reinfection.Applied microbiology and biotechnology · 2024Review
- Direct comparison of spatial transcriptional heterogeneity across diverse Bacillus subtilis biofilm communities.Nature communications · 2023Article
- Article
- Growth in a biofilm promotes conjugation of amSphere · 2023Article
- Transcriptional profilingMicrobiology (Reading, England) · 2023Article
- Biofilm of Klebsiella pneumoniae minimize phagocytosis and cytokine expression by macrophage cell line.AMB Express · 2022Article
- Polymicrobial Biofilm Organization ofInternational journal of molecular sciences · 2022Article
- Strategies to prevent, curb and eliminate biofilm formation based on the characteristics of various periods in one biofilm life cycle.Frontiers in cellular and infection microbiology · 2022Review
- Peptidomimetics as Potential Anti-Virulence Drugs Against Resistant Bacterial Pathogens.Frontiers in microbiology · 2022Review
- Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSurface-associated communities of bacteria, known as biofilms, play a critical role in the persistence and dissemination of bacteria in various environments. Biofilm development is a sequential dynamic process from an initial bacterial adhesion to a three-dimensional structure formation, and a subsequent bacterial dispersion. Transitions between these different modes of growth are governed by complex and partially known molecular pathways.
resultsUsing RNA-seq technology, our work provided an exhaustive overview of the transcriptomic behavior of the opportunistic pathogen Klebsiella pneumoniae derived from free-living, biofilm and biofilm-dispersed states. For each of these conditions, the combined use of Z-scores and principal component analysis provided a clear illustration of distinct expression profiles. In particular, biofilm-dispersed cells appeared as a unique stage in the bacteria lifecycle, different from both planktonic and sessile states. The K-means cluster analysis showed clusters of Coding DNA Sequences (CDS) and non-coding RNA (ncRNA) genes differentially transcribed between conditions. Most of them included dominant functional classes, emphasizing the transcriptional changes occurring in the course of K. pneumoniae lifestyle transitions. Furthermore, analysis of the whole transcriptome allowed the selection of an overall of 40 transcriptional signature genes for the five bacterial physiological states.
conclusionsThis transcriptional study provides additional clues to understand the key molecular mechanisms involved in the transition between biofilm and the free-living lifestyles, which represents an important challenge to control both beneficial and harmful biofilm. Moreover, this exhaustive study identified physiological state specific transcriptomic reference dataset useful for the research community.
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