ArticleScientific reports2016
The DmtA methyltransferase contributes to Aspergillus flavus conidiation, sclerotial production, aflatoxin biosynthesis and virulence.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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Who cites it
67 citing papers in PubMed, 103 citations in OpenAlex.
- Giant transposable elements drive genetic diversification iniScience · 2026Article
- Aflrpn4 Represents a Promising Target for MitigatingToxins · 2026Article
- 5mC and 6mA DNA Methylation in the Fungal Kingdom: From Genome Defense to Epigenetic Regulation.Epigenomes · 2026Review
- Identification and Characterization of a Red-Light Sensor FphA inInternational journal of molecular sciences · 2026Article
- COMPASS subunit Bre2 regulates chromatin remodeler Arp9 to control Aspergillus flavus aflatoxin synthesis and virulence.Nature communications · 2026Article
- Role of theToxins · 2025Article
- STA regulates succinylated AflM triggered by SCS to contribute to aflatoxin biosynthesis through the Ach1.Virulence · 2025Article
- From the bench to the reactor: engineered filamentous fungi for biochemical and biomaterial production.Biotechnology for biofuels and bioproducts · 2025Review
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- Considerations for Domestication of Novel Strains of Filamentous Fungi.ACS synthetic biology · 2025Review
- The Autophagy-Related Protein ATG8 Orchestrates Asexual Development and AFB1 Biosynthesis inJournal of fungi (Basel, Switzerland) · 2024Article
- Chitin Deacetylase Homologous GeneToxins · 2024Article
- AflaILVB/G/I and AflaILVD are involved in mycelial production, aflatoxin biosynthesis, and fungal virulence inFrontiers in cellular and infection microbiology · 2024Article
- Regulation of Fungal Morphogenesis and Pathogenicity ofJournal of fungi (Basel, Switzerland) · 2023Article
- DNA methylation-dependent epigenetic regulation ofaBIOTECH · 2023Article
- The model diatom Phaeodactylum tricornutum provides insights into the diversity and function of microeukaryotic DNA methyltransferases.Communications biology · 2023Article
- Set2 family regulates mycotoxin metabolism and virulence via H3K36 methylation in pathogenic fungusVirulence · 2022Article
- The histone code of the fungal genusMicrobial genomics · 2022Article
- GTPase Rac Regulates Conidiation, AFB1 Production and Stress Response in Pathogenic FungusToxins · 2022Article
- Post-translational modifications drive secondary metabolite biosynthesis in Aspergillus: a review.Environmental microbiology · 2022Review
7 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA methylation is essential for epigenetic regulation of gene transcription and development in many animals, plants and fungi. We investigated whether DNA methylation plays a role in the development and secondary metabolism of Aspergillus flavus, identified the DmtA methyltransferase from A. flavus, and produced a dmtA knock-out mutant by replacing the dmtA coding sequence with the pyrG selectable marker. The A. flavus dmtA null mutant lines produced white fluffy mycelium in liquid medium, and displayed a slightly flavescent conidial pigmentation compared with the normal yellow of the wild-type strain when grown on agar. The ΔdmtA lines exhibited decreased conidiation and aflatoxin (AF) biosynthesis, compared with the wild-type line, suggesting that the DmtA knock-out affected the transcriptional level of genes in the AF cluster. In particular, sclerotia development and host colonization were altered in the dmtA null mutants. Green fluorescent protein tagging at the C-terminus of DmtA showed that DmtA localized to the nucleus and cytoplasm. DNA methylation content measurements in the dmtA mutants revealed no widespread DNA methylation in the mutants or wild-type lines. Thus, our findings suggest that DmtA, apart from being a C-5 cytosine methyltransferase in A. flavus, contributes to asexual development, aflatoxin biosynthesis, sclerotial production and virulence.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.