ArticleJournal of proteome research2016
Extraction, Enrichment, Solubilization, and Digestion Techniques for Membrane Proteomics.
Article in Journal of proteome research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed.
- Lyophilization Prior to Homogenisation and Extraction Increases Membrane Protein Detection in Gram-Negative Bacterial Proteomic Analyses.Proteomes · 2026Article
- Proteomic Evidence Indicates Ultrasound-Assisted Extraction Enhances Recovery of Membrane-Associated Proteins inJournal of agricultural and food chemistry · 2026Article
- Comparative performance of traditional and commercial DNA extraction methods for fish gut microbiota analysis.Molecular biology reports · 2026Article
- Biomimetic liposomes in drug delivery: from design mechanisms to applications.Chemical Society reviews · 2026Review
- Development of a Novel Immunoprecipitation Method for Extracting Monoclonal Antibodies From Brain Tissue and Its Application to Assessing In Vivo Brain Penetration in Mouse via Liquid Chromatography-Mass Spectrometry.Biomedical chromatography : BMC · 2025Article
- Protein Sample Preparation for Bottom-Up, Label-Free Quantitative Proteomics of Adipose Tissue.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Enhanced Electrophoretic Depletion of Sodium Dodecyl Sulfate with Methanol for Membrane Proteome Analysis by Mass Spectrometry.Proteomes · 2024Article
- Top-down proteomics.Nature reviews. Methods primers · 2024Article
- Overview and considerations in bottom-up proteomics.The Analyst · 2023Review
- Simple But Efficacious Enrichment of Integral Membrane Proteins and Their Interactions for In-Depth Membrane Proteomics.Molecular & cellular proteomics : MCP · 2022Article
- Dynamic Distribution of ASIC1a Channels and Other Proteins within Cells Detected through Fractionation.Membranes · 2022Article
- A fast and reliable method for monitoring genomic instability in the model organism Caenorhabditis elegans.Archives of toxicology · 2021Article
- Strategies for Rapid Identification ofFrontiers in cellular and infection microbiology · 2021Article
- Neuronal subclass-selective proteomic analysis in Caenorhabditis elegans.Scientific reports · 2020Article
- Protein Expression and Functional Relevance of Efflux and Uptake Drug Transporters at the Blood-Brain Barrier of Human Brain and Glioblastoma.Clinical pharmacology and therapeutics · 2020Article
- Global variability analysis of mRNA and protein concentrations across and within human tissues.NAR genomics and bioinformatics · 2020Article
- MS1 ion current-based quantitative proteomics: A promising solution for reliable analysis of large biological cohorts.Mass spectrometry reviews · 2019Review
- Review
- Triflic Acid Treatment Enables LC-MS/MS Analysis of Insoluble Bacterial Biomass.Journal of proteome research · 2018Article
- Top-Down Proteomics: Ready for Prime Time?Analytical chemistry · 2018Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The importance of membrane proteins in biological systems is indisputable; however, their amphipathic nature makes them difficult to analyze. In this study, the most popular techniques for extraction, enrichment, solubilization, and digestion are compared, resulting in an overall improved workflow for the insoluble portion of Saccharomyces cerevisiae cell lysate. Yeast cells were successfully lysed using a French press pressure cell at 20 000 psi, and resulting proteins were fractionated prior to digestion to reduce sample complexity. The proteins were best solubilized with the addition of ionic detergent sodium deoxycholate (1%) and through the application of high-frequency sonication prior to a tryptic digestion at 37 °C. Overall, the improved membrane proteomic workflow resulted in a 26% increase in membrane protein identifications for baker's yeast. In addition, more membrane protein identifications were unique to the improved protocol. When comparing membrane proteins that were identified in the improved protocol and the standard operating procedure (176 proteins), 93% of these proteins were present in greater abundance (higher intensity) when using the improved method.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.