Evidence map›Paper›PMID 26978347›Full record

ArticleInternational journal of molecular sciences2016

Co-Expression and Co-Localization of Cartilage Glycoproteins CHI3L1 and Lubricin in Osteoarthritic Cartilage: Morphological, Immunohistochemical and Gene Expression Profiles.

Marta Anna Szychlinska, Francesca Maria Trovato, Michelino Di Rosa, Lucia Malaguarnera, Lidia Puzzo, Rosy Leonardi, Paola Castrogiovanni, Giuseppe Musumeci

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
48citing papers in PubMed, 2 pooled it
8.1field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

48 citing papers in PubMed, 2 syntheses or guidelines pooled it, 75 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Marta Anna SzychlinskaDepartment of Biomedical and Biotechnological Sciences, Human Anatomy and Histology Section, School of Medicine, University of Catania, Via S. Sofia 87, 95123 Catania, Italy. mszychlinska@unict.it.
Francesca Maria TrovatoDepartments of Clinical and Experimental Medicine, Internal Medicine Division, School of Medicine, University of Catania, 95123 Catania, Italy. trovatofrancesca@gmail.com.
Michelino Di RosaDepartment of Biomedical and Biotechnological Sciences, Pathology Section, School of Medicine, University of Catania, 95125 Catania, Italy. mdirosa@unict.it.
Lucia MalaguarneraDepartment of Biomedical and Biotechnological Sciences, Pathology Section, School of Medicine, University of Catania, 95125 Catania, Italy. lucmal@unict.it.
Lidia PuzzoDepartment of Medical and Surgical Sciences and Advanced Technologies, Anatomic Pathology Section, School of Medicine, University of Catania, 95123 Catania, Italy. lipuzzo@unict.it.
Rosy LeonardiDepartment of Medical and Surgical Science, Section of Dentistry, University of Catania, 95123 Catania, Italy. rleonard@unict.it.
Paola CastrogiovanniDepartment of Biomedical and Biotechnological Sciences, Human Anatomy and Histology Section, School of Medicine, University of Catania, Via S. Sofia 87, 95123 Catania, Italy. pacastro@unict.it.
Giuseppe MusumeciDepartment of Biomedical and Biotechnological Sciences, Human Anatomy and Histology Section, School of Medicine, University of Catania, Via S. Sofia 87, 95123 Catania, Italy. g.musumeci@unict.it.
University of Catania · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Osteoarthritis is the most common human arthritis characterized by degeneration of articular cartilage. Several studies reported that levels of human cartilage glycoprotein chitinase 3-like-1 (CHI3L1) are known as a potential marker for the activation of chondrocytes and the progression of Osteoarthritis (OA), whereas lubricin appears to be chondroprotective. The aim of this study was to investigate the co-expression and co-localization of CHI3L1 and lubricin in normal and osteoarthritic rat articular cartilage to correlate their modified expression to a specific grade of OA. Samples of normal and osteoarthritic rat articular cartilage were analyzed by the Kellgren-Lawrence OA severity scores, the Kraus' modified Mankin score and the Histopathology Osteoarthritis Research Society International (OARSI) system for histomorphometric evaluations, and through CHI3L1 and lubricin gene expression, immunohistochemistry and double immuno-staining analysis. The immunoexpression and the mRNA levels of lubricin increased in normal cartilage and decreased in OA cartilage (normal vs. OA, p < 0.01). By contrast, the immunoexpression and the mRNA levels of CHI3L1 increased in OA cartilage and decreased in normal cartilage (normal vs. OA, p < 0.01). Our findings are consistent with reports suggesting that these two glycoproteins are functionally associated with the development of OA and in particular with grade 2/3 of OA, suggesting that in the future they could be helpful to stage the severity and progression of the disease.

Indexed as

AnimalsCartilage, ArticularChitinase-3-Like Protein 1Disease Models, AnimalGene Expression RegulationGlycoproteinsHumansMaleOsteoarthritisProteoglycan 4ProteoglycansRatsRats, WistarCHI3L1 protein, ratChitinase-3-Like Protein 1GlycoproteinsPRG4 protein, ratProteoglycan 4Proteoglycansanterior cruciate ligament transection (ACLT)CHI3L1ImmunohistochemistryLubricinmRNAOsteoarthritis

Identifiers

PMID26978347
PMCPMC4813220
OpenAlexW2295844066

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.