ArticlePloS one2016
Fragile Sites of 'Valencia' Sweet Orange (Citrus sinensis) Chromosomes Are Related with Active 45s rDNA.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
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- Comprehensive mapping of molecular cytogenetic markers in pitaya (Frontiers in plant science · 2024Article
- The Chromosome Number and rDNA Loci Evolution inInternational journal of molecular sciences · 2022Article
- Molecular and Cytogenetic Analysis of rDNA Evolution in Crepis Sensu Lato.International journal of molecular sciences · 2022Article
- Interstitial Telomeric-like Repeats (ITR) in Seed Plants as Assessed by Molecular Cytogenetic Techniques: A Review.Plants (Basel, Switzerland) · 2021Review
- B Chromosomes in GenusPlants (Basel, Switzerland) · 2021Review
- Localization and characterization of Citrus centromeres by combining half-tetrad analysis and CenH3-associated sequence profiling.Plant cell reports · 2020Article
- Integrated Karyotypes of Diploid and Tetraploid Carrizo Citrange (Frontiers in plant science · 2020Article
- DetectingInternational journal of molecular sciences · 2019Article
- Molecular cytogenetic analysis of genome-specific repetitive elements in Citrus clementina Hort. Ex Tan. and its taxonomic implications.BMC plant biology · 2019Article
- 45S rDNA sites in meiosis of Lolium multiflorum Lam.: variability, non-homologous associations and lack of fragility.Protoplasma · 2019Article
- Karyotype Analysis of Diploid and Spontaneously Occurring Tetraploid Blood Orange [Frontiers in plant science · 2019Article
- Relationship between epigenetic marks and the behavior of 45S rDNA sites in chromosomes and interphase nuclei of Lolium-Festuca complex.Molecular biology reports · 2018Article
- Karyotype reshufflings of Festuca pratensis × Lolium perenne hybrids.Protoplasma · 2018Article
- Karyotype heterogeneity in Philodendron s.l. (Araceae) revealed by chromosome mapping of rDNA loci.PloS one · 2018Article
- High and uneven levels of 45S rDNA site-number variation across wild populations of a diploid plant genus (Anacyclus, Asteraceae).PloS one · 2017Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Citrus sinensis chromosomes present a morphological differentiation of bands after staining by the fluorochromes CMA and DAPI, but there is still little information on its chromosomal characteristics. In this study, the chromosomes in 'Valencia' C. sinensis were analyzed by fluorescence in situ hybridization (FISH) using telomere DNA and the 45S rDNA gene as probes combining CMA/DAPI staining, which showed that there were two fragile sites in sweet orange chromosomes co-localizing at distended 45S rDNA regions, one proximally locating on B-type chromosome and the other subterminally locating on D-type chromosome. While the chromosomal CMA banding and 45S rDNA FISH mapping in the doubled haploid line of 'Valencia' C. sinensis indicated six 45S rDNA regions, four were identified as fragile sites as doubled comparing its parental line, which confirmed the cytological heterozygosity and chromosomal heteromorphisms in sweet orange. Furthermore, Ag-NOR identified two distended 45S rDNA regions to be active nucleolar organizing regions (NORs) in diploid 'Valencia' C. sinensis. The occurrence of quadrivalent in meiosis of pollen mother cells (PMCs) in 'Valencia' sweet orange further confirmed it was a chromosomal reciprocal translocation line. We speculated this chromosome translocation was probably related to fragile sites. Our data provide insights into the chromosomal characteristics of the fragile sites in 'Valencia' sweet orange and are expected to facilitate the further investigation of the possible functions of fragile sites.
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