ArticleMolecular metabolism2016
High-fat diet reprograms the epigenome of rat spermatozoa and transgenerationally affects metabolism of the offspring.
Article in Molecular metabolism, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 196 papers, 2 of them syntheses that pooled it.
What it found
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Who cites it
196 citing papers in PubMed, 2 syntheses or guidelines pooled it, 413 citations in OpenAlex.
- Pooled it
- OBEDIS Core Variables Project: European Expert Guidelines on a Minimal Core Set of Variables to Include in Randomized, Controlled Clinical Trials of Obesity Interventions.Obesity facts · 2020Guideline
- Paternal cytokine administration alters sperm small ncRNAs and offspring brain and behavior.Molecular psychiatry · 2026Article
- Review
- Dietary restriction induces persistent multigenerational phenotypic effects in Phytoseiulus persimilis.Pest management science · 2026Article
- Paternal folate deficiency induces hepatic insulin resistance in offspring mice.Nutrition & diabetes · 2026Article
- Paternal Circadian Disruption Impairs Offspring Cognition via Sperm microRNAs.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Blood DNA methylation of EIF5A and TGIF1 is associated with adipose tissue health and metabolic outcomes in obesity: a multi-cohort study.Clinical epigenetics · 2026Article
- The Role of Fathers in the Intergenerational Transmission of Obesity.Current obesity reports · 2026Review
- A Review of the Effects of Maternal and Paternal Obesity on Neurodevelopmental Disorders and Related Neurobiology in Rodent and Human Offspring.Obesity reviews : an official journal of the International Association for the Study of Obesity · 2026Review
- Effects of obesity on DNA methylation and DNA methyltransferases in the reproductive system.Histochemistry and cell biology · 2026Review
- Sperm sncRNA networks are synchronized by a coordination between adverse childhood experiences (ACE) and current stress.Scientific reports · 2026Article
- Cross-Generational Integration of Exercise and Nutritional Encoding in Offspring Adipose Genomics.International journal of molecular sciences · 2026Review
- Male obesity causes adipose mitochondrial dysfunction in FNature communications · 2026Article
- Maternal Overnutrition and Fetal Programming: Long-Term Metabolic, Cognitive, and Epigenetic Consequences.Cells · 2026Review
- Paternal Microplastic Exposure Alters Sperm Small Noncoding RNAs and Affects Offspring Metabolic Health in Mice.Journal of the Endocrine Society · 2026Article
- The Correlation betweenInternational journal of fertility & sterility · 2026Article
- Environmental and Genetic Perturbations of the Sperm Epigenome.Advances in experimental medicine and biology · 2026Review
- Phthalate exposure to adult mice alters non-coding RNAs in cauda epididymosomes.Environmental pollution (Barking, Essex : 1987) · 2025Article
- Toward Understanding and Halting Legacies of Trauma.Biological psychiatry · 2025Review
136 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
14 authors at 2 institutions in 2 countries.
Funding
Abstract
objectivesChronic and high consumption of fat constitutes an environmental stress that leads to metabolic diseases. We hypothesized that high-fat diet (HFD) transgenerationally remodels the epigenome of spermatozoa and metabolism of the offspring.
methodsF0-male rats fed either HFD or chow diet for 12 weeks were mated with chow-fed dams to generate F1 and F2 offspring. Motile spermatozoa were isolated from F0 and F1 breeders to determine DNA methylation and small non-coding RNA (sncRNA) expression pattern by deep sequencing.
resultsNewborn offspring of HFD-fed fathers had reduced body weight and pancreatic beta-cell mass. Adult female, but not male, offspring of HFD-fed fathers were glucose intolerant and resistant to HFD-induced weight gain. This phenotype was perpetuated in the F2 progeny, indicating transgenerational epigenetic inheritance. The epigenome of spermatozoa from HFD-fed F0 and their F1 male offspring showed common DNA methylation and small non-coding RNA expression signatures. Altered expression of sperm miRNA let-7c was passed down to metabolic tissues of the offspring, inducing a transcriptomic shift of the let-7c predicted targets.
conclusionOur results provide insight into mechanisms by which HFD transgenerationally reprograms the epigenome of sperm cells, thereby affecting metabolic tissues of offspring throughout two generations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.